不育男性生殖道沙眼衣原体、解脲支原体感染与精子凋亡关系

Relationship between sperm apoptosis and genital tract chlamydia trachomatis and ureplasma urealyticum infections in infertile males

目的 :探讨不育男性生殖道沙眼衣原体 ( CT)、解脲支原体 ( UU)感染与精子凋亡的关系。方法 :采用流式细胞术 ( FCM)检测生殖道 UU、CT感染者和未感染者精子凋亡情况。结果 :CT、UU阳性和阴性组精子凋亡率 ( PAS)分别为 ( 1 4 .54± 3 .90 ) %、( 8.3 7± 6.3 0 ) %、( 1 5.2 6± 6.56) %、( 9.2 5± 4.0 3 ) % ,CT+UU双阳性精子凋亡率高达( 2 2 .2 1± 6.52 ) % ,各组与正常生育组比较 ,差异非常显著 ( P<0 .0 1 )。阳性组与相应阴性组比较差异显著 ( P<0 .0 5)。结论 :生殖道 UU、CT感染可明显增加精子凋亡 ,提示引起男性不育的诸多因素可能因精子细胞凋亡所致。

Objective: To investigate the relationship between sperm apoptosis and genital tract chlamydia trachomatis (CT) and ureplasma urealyticum (UU) infections in infertile males. Methods: Percentages of apoptotic sperm (PAS) in patients with CT and UU infections were tested by flow cytometry(FCM) with FITC annexin V/PI staining Results: Sperm apoptosis had happened in male infertile patients with CT and UU infections. The PAS in CT and UU positive and negative groups were (14.54± 3.90)%, (8.37±6.30)%, (15.26±6.56)%, (9.25±4.03)%, respectively. The differences between CT/UU positive and negative groups were significant ( P <0.05). The PAS in both CT and UU positive group was higher(22.21±6.52)% than that of normal control (5.50±2.08)( P <0.01) Conclusion: There was vary close relationship between sperm apoptosis and genital tract CT and UU infections in infertile males. The genital tract CT and UU infections may lead to marked increasing of the sperm apoptosis.