不同浓度EPA和DHA对C2C12成肌细胞增殖及凋亡的影响

Effects of Different Concentrations of EPA and DHA on Proliferation and Apoptosis of C2C12 Myoblast

本试验旨在研究二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)对体外培养的C2C12成肌细胞增殖和凋亡的影响。用不同终浓度DHA和EPA(0、6.25、12.5、25、50、100μmol/L)分别作用C2C12细胞12、24、48 h后,用CCK-8法检测细胞的增殖情况;用不同终浓度DHA和EPA(0、50、100μmol/L)分别作用C2C12细胞48 h后,用TUNEL法检测细胞凋亡情况。结果表明:终浓度为50、100μmol/L的EPA处理细胞48 h,C2C12细胞增殖能力受到显著抑制,但不同浓度DHA处理组细胞的增殖能力无明显变化。此外,EPA处理C2C12细胞后,TUNEL法显示凋亡细胞数增加,且100μmol/L的EPA处理组细胞凋亡最明显。然而,与对照相比,DHA处理组细胞无明显凋亡。上述结果表明,EPA能抑制C2C12成肌细胞增殖并促进其凋亡,而DHA对细胞增殖和凋亡无明显影响。

This experiment was conducted to investigate the effects of eicosapentaenoic acid （EPA） and docosahexaenoic acid （DHA） on cell proliferation and apoptosis of C2C12 myoblasts. We treated C2C12 myoblasts with different concentrations of DHA and EPA （0, 6.25, 12.5, 25, 50 and 100 μ mol/L, respectively） for 12 h, 24 h and 48 h, and monitored the cell lines for alterations in proliferation with the Cell Counting Kit-8. In addition, we detected the C2C12 apoptosis under treatment with different concentrations of DHA and EPA （0, 50 and 100 μ mol/L） for 48 h by using the TUNEL assay. The results showed that the C2C12 proliferation was significantly inhibited after the treatment with 50 and 100 g mol/L EPA for 48 h. However, there was no obvious difference in the proliferation of the C2C12 treated with various concentrations of DHA. In addition, the TUNEL assay showed that treatment with EPA increased the number of apoptotic cells, and the cellular apoptosis was more obvious under treatment with 100 g mol/L EPA. Compared with the control group, no obvious cellular apoptosis was observed under treatment with DHA. These results indicate that, EPA can inhibit C2C12 myoblast proliferation and promote its apoptosis, while DHA have no obvious effects on cell proliferation and apoptosis.