愈银片的HPLC指纹图谱研究

Study on the HPLC Fingerprint Chromatogram of Yuyin Tablet

目的:建立愈银片的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Inertsil ODS-3,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为230 nm,柱温为30℃,进样量为10μL。以槲皮素为参照物,测定10批愈银片的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行共有峰指认和相似度评价。结果:10批愈银片的HPLC图谱有39个共有峰,相似度均>0.96。经验证,10批样品HPLC图谱与对照指纹图谱具有较好的一致性。结论:该研究所建指纹图谱可为愈银片的鉴别和质量评价提供参考。

OBJECTIVE： To establish the HPLC fingerprint for chromatogram Yuyin tablet. METHODS： HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-0.1% phosphoric acid （gradient elution） at a flow rate of 1.0 mL/min, detection wavelength was 230 nm, column temperature was 30 ℃, and injection volume was 10 μL. Using quercetin as reference, the HPLC profiles of 10 batches of Yuyin tablet were detemined, Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition） was used for the common peaks iolentification and similarity evaluation. RESULTS： There were 39 common peaks in the HPLC profiles of the 10 batches of Yuyin tablet, similarity degrees were higher than 0.96. It was proved that the HPLC profiles and control fingerprint profile of 10 batches of samples had good consistency. CONCLUSIONS： The established fingerprint chromatogram can provide reference for the identification and quality evaluation of Yuyin tablet.