摘要
目的通过康莱特注射液作用于人胰腺癌细胞株PANC-1,观察其对PANC-1细胞增殖和凋亡的影响,并进一步探讨其作用机制。方法倒置显微镜及电镜观察80μL·m L^(-1)康莱特注射液作用PANC-1细胞72 h后细胞形态改变;采用MTT比色法检测不同浓度(10、20、40、60、80、100μL·m L^(-1))康莱特注射液对PANC-1细胞生长增殖抑制作用的影响;同时采用流式细胞术检测不同浓度(10、20、40、60、80、100μL·m L^(-1))康莱特注射液对PANC-1细胞周期的影响。结果细胞形态学检查发现80μL·m L^(-1)康莱特注射液处理PANC-1细胞72 h后细胞生长稀疏,细胞质透亮度下降,线粒体等细胞器功能障碍,细胞核功能上受到一定的抑制;不同浓度康莱特注射液作用于PANC-1细胞株不同时段(24、48、72 h)后肿瘤细胞生长受到了一定的抑制且呈现时间和剂量依赖性;不同浓度康莱特注射液处理PANC-1胰腺癌细胞72 h后PANC-1细胞周期均阻滞于G2/M期,康莱特注射液可剂量依赖性的诱导PANC-1细胞凋亡。结论康莱特注射液能够有效抑制人胰腺癌细胞株PANC-1在体外的生长,其机制可能是通过抑制细胞增殖,促进肿瘤细胞的凋亡而实现,有望成为胰腺癌的有效治疗药物。
Objective To investigate the effects of kanglaite injection on the proliferation and apoptosis of pancreatic cancer PANC-1 cells. Methods Different concentration of sodium kanglaite injection( 10,20,40,60,80,100μL·m L^(-1)) were adder to PANC-1 cells for 24,48,72 h. Inverted microscope and electron microscope were used to observe the change of cell morphology of 80 μL·m L^(-1)kanglaite injection of PANC-1 cells after 72 h; the MTT assay was performed to reveal the inhibitory effect on cell proliferation; cell cycle was tested by flow cytometry. Results Cell morphology examination found cell growth and sparse cytoplasm brightness decreased,mitochondria dysfunction,nuclear function was inhibited,after 80 μL·m L^(-1)kanglaite injection treatment of PANC-1 cells of 72 h;kanglaite injection with different concentration treatment inhibited the proliferation in a dose and time-dependent manner; different concentration of kanglaite injection cocultured PANC-1 cells after 72 h,most of PANC-1 cell cycle arrested in G2/M phase,kanglaite injection could induce the apoptosis of PANC-1 cells dose dependently. Conclusion Kanglaite injection can effectively inhibit the growth of the human pancreatic cancer PANC-1 cells in vitro,and its mechanism may be through inhibition of cell proliferation,promote apoptosis of tumor cells and implementation,is expected to become the effective drugs for the treatment of pancreatic carcinoma.
出处
《肿瘤基础与临床》
2016年第5期369-372,共4页
journal of basic and clinical oncology
关键词
胰腺癌
康莱特注射液
细胞增殖
细胞凋亡
pancreatic carcinoma
kanglaite injection
cell proliferation
cell apoptosis