宫腔粘连子宫内膜组织中ArhGAP29、E-cadherin的表达及意义

Expression and significance of ArhGAP29 and E-cadherin in intrauterine adhesions of endometrium

目的探讨ArhGAP29和E-cadherin在宫腔粘连(intrauterine adhesions,IUAs)子宫内膜组织中的表达及其临床意义,分析ArhGAP29、E-cadherin在IUAs发病中的作用机制。方法采用免疫组化PV 9000两步法检测IUAs中ArhGAP29、E-cadherin的表达,分析其与IUAs的临床病理特征关系以及ArhGAP29、E-cadherin的相关性。结果(1)ArhGAP29、E-cadherin在正常子宫内膜中表达的免疫组化反应评分(immunoreactive score,IRS)高于IUAs患者,差异有统计学意义(P=0.017,P=0.004);(2)中度IUAs患者子宫内膜中ArhGAP29、E-cadherin表达的IRS高于重度IUAs患者,差异有统计学意义(P=0.020,P=0.026);非闭经IUAs患者子宫内膜ArhGAP29、E-cadherin表达的IRS高于闭经IUAs患者,差异有统计学意义(P=0.019,P=0.031);(3)ArhGAP29表达降低与E-cadherin低表达之间呈显著正相关(r=0.725,P<0.001)。结论 ArhGAP29、E-cadherin在IUAs子宫内膜中表达降低,其下降程度与IUAs严重程度有关,ArhGAP29、E-cadherin降低可能参与IUAs的形成。

Purpose To investigate the expression and sig- nificance of ArhGAP29 and E-cadherin in endometrial tissue of intrauterine adhesions （IUAs） and to explore their roles in the pathogenesis of IUAs. Methods The expression of ArhGAP29 and E-cadherin was detected by immunohistochemical PV 9000 two-step method. The correlation between ArhGAP29 and E-cad- herin expression and clinical features was analyzed. Results （ 1 ） The immunoreactive score （IRS） of ArhGAP29 and E-cad- herin in normal endometrial tissue were higher than those in IUAs endometrial tissue （P =0. 017, P =0. 004）. （2） IRS of Arh- GAP 29 and E-cadherin in moderate IUAs patients were higher than that in severe IUAs patients （P = 0. 020, P = 0. 026）. InIUAs patients without amenorrhea, the IRS of ArhGAP29 and E- cadherin were higher （P =0. 019, P =0. 031 ） than that in IUAs patients with amenon-hea. （3） The decrease of ArhGAP29 ex- pression had a significantly parallel relationship with the negative expression of E-cadherin （ r = 0. 725, P 〈 0. 001 ）. Conclusion The expression of ArhGAP29 and E-cadherin decreases in en- dometrial tissue of IUAs patients, which is related with degree of IUAs severity. ArhGAP29 and E-cadherin may participate in the IUAs formation.