子宫颈癌中高危型HPV感染与c-jun和c-fos蛋白表达的关系

Relationship of high-risk human papillomavirus infection and c-jun and c-fos expression in cervical carcinoma

目的探讨子宫颈癌中高危型人乳头状瘤病毒(high-risk human papilloma virus,HR-HPV)感染与c-jun和c-fos蛋白表达的关系及临床意义。方法采用Cervista技术对70例子宫颈鳞状细胞癌(cervical squmaous cell carcinoma,CSCC)、60例子宫颈上皮内病变(cervical intraepithelial neoplasia,CIN)及20例慢性子宫颈炎行HR-HPV检测,同时应用免疫组化SP法检测AP-1通路重要组份c-jun和c-fos蛋白的表达,分析HR-HPV感染与c-jun和c-fos蛋白表达的相关性及与子宫颈癌临床病理特征的关系。结果 70例CSCC中69例HR-HPV呈阳性,阳性率98.6%(69/70),其中A9组阳性占比最高,达85.5%(59/69)。70例CSCC中c-jun和c-fos的阳性率分别为80%(56/70)、85.7%(60/70),30例CIN2+CIN3中的阳性率均为70%(21/30),30例CIN1中的阳性率分别为20%(6/30)、23.3%(7/30),20例慢性子宫颈炎中的阳性率均为0。CSCC组与CIN组及慢性子宫颈炎组相比差异有统计学意义(P<0.05)。c-jun、c-fos阳性率在CSCC的临床分期、组织学分级等分组中差异有统计学意义(P<0.05);CSCC中HR-HPV阳性与c-jun、c-fos的表达均呈正相关(P<0.01)。结论 CSCC中HR-HPV感染具有显著的亚型特点,且与c-jun和c-fos表达呈正相关,提示HR-HPV感染后AP-1通路活化可能与肿瘤的发生、发展相关。

Purpose To investigate the relationship and clinical significance of high-risk human papillomavirus infection and expression of c-jun and c-los protein in cervical carcinoma. Methods Cervista HR-HPV testing, immunohistochemical SP method were employed to detect the infection of HR-HPV, c-jun and c-fos protein in 70 cases of CSCC, 60 cases of cervical in-traepithelial neoplasia and 20 cases of chronic cervicitis. The correlation between c-jun and c-los protein expression and of HR-HPV infection was analyzed. Results Of the 70 cases of CSCC, the positive HR-HPV was 69 cases, the positive rate in HR-HPV in A9 group was the highest （85%, 59/69）. The positive expression of c-jun and c-los were80% （56/70）,85.7% （60/70） in 70 cases CSCC, 70% （21/30） and 70% （21/30） in 30 cases of cervical high grade intraepithelial neo- plasia, and 20% （6/30）, 23.3% （7/30） in 30 cases cervical low grade intraepithelial neoplasia, but the positive expression rates of c-jun and c-fos in the 20 cases of chronic cervicitis were 0. Expression of c-jun and c-fos in cervical cancer group was higher than that in the chronic cervicitis group and low grade in- traepithelial neoplasia （P 〈 0. 05 ）. The expression of c-jun and c-los was statistically significant in different groups of clinicalstage and pathologic grading in the CSCC （ P 〈 0. 05 ）. Addition- ally, the expression of c-jun and c-fos was positively correlated with the infection of HR-HPV in CSCC （P 〈0. 01）. Conclusion The infection of HR-HPV has significant subtype-specific and has a positive correlation with the expression of c-jun and c-fos in CSCC, which suggests that AP-1 pathway activation after HR- HPV infection may be associated with the occurrence and devel- opment of cervical carcinoma.