大疱性类天疱疮患者疱液中趋化因子受体5和可诱导共刺激分子的检测

Detection of C-X-C chemokine receptor type 5 and inducible costimulator in blister fluid of patients with bullous pemphigoid

目的检测CX—C趋化因子受体5（CXCR5）和可诱导共刺激分子（ICOS）在大疱性类天疱疮（BP）患者疱液中的水平，探讨其在BP发病机制中的意义。方法收集15例BP患者疱液为实验组，15例Ⅱ度烧伤患者疱液为对照组，采用酶联免疫吸附测定（ELISA）方法分别检测CXCR5及ICOS在两组疱液中的水平。结果实验组及对照组疱液中CXCR5水平分别为（219±145．31）、（147±23．83）ng／L，差异有统计学意义（t=4．577，P〈0．05），但疱液中ICOS水平差异无统计学意义[（30．18±14．86）比（21．43±5．32）ng／L，t=1．628，P〉0．05]。结论CXCR5的表达可能与BP发病有一定关系，而ICOS与BP发病的关系尚需进一步研究。

Objective To detect levels of C-X-C chemokine receptor type 5 （CXCR5） and inducible costimulator （ICOS） in blister fluid of patients with bullous pemphigoid （BP）, and to explore their significance in the pathogenesis of BP. Methods Blister fluid samples were collected from 15 patients with BP （experimental group） and 15 patients with second-degree burns （control group）. Enzymelinked immunosorbent assay （ELISA） was performed to detect the levels of CXCR5 and ICOS in the 2 groups. Results The level of CXCR5 was significantly higher in the experimental group than in the control group （219 ± 145.31 vs. 147 ± 23.83 ng/L, t = 4.577, P 〈 0.05）, while no significant difference in the ICOS level was observed between the 2 groups （30.18 ± 14.86 vs. 21.43 ± 5.32 ng/L, t = 1.628, P 〉 0.05）. Conclusion The expression of CXCR5 may be associated with the occurrence of BP, but further researches are needed to determine the relationship between ICOS and the occurrence of BP.