大豆GmMP基因的功能预测及表达分析

Functional Prediction and Expression Analysis of GmMP in Soybean

通过在大豆基因组数据库中检索拟南芥AtMP(ARF5)在大豆中的同源基因,获得了GmMP基因序列。对GmMP基因编码的氨基酸序列及启动子序列进行生物信息学分析,结果表明:GmMP基因CDS序列全长2 802 bp,编码933个氨基酸。GmMP编码的蛋白为疏水性蛋白。结构域分析表明:GmMP含有B3和AUXIN RESPONSE FACTOR结构域,同时该基因是ARF家族的成员。GmMP预测的启动子区域含有与激素、胁迫、光应答、生物钟调控和转录因子结合相关的顺式作用元件。系统进化分析表明MP在豆科植物进化过程中比较保守。组织特异性表达分析结果显示GmMP在叶片中表达量最低,在茎尖中表达量最高,推测其可能参与生长素的代谢途径。

GmMP gene sequence was obtained by searching the homologous gene of AtMP in the soybean genome database. Bioinformaties analysis of amino acid sequence encoded by GmMP and promoter sequences showed that the full-length CDS se- quence was 2 802 bp, encoding 933 amino acids protein. Structure domains analysis indicated that GmMP contained B3 and AUXIN RESPONSE FACTOR domain, belong to B3 and AUXIN RESPONSE superfamily. Cis-elements associated with hor- mones, stresses, light responses, circadian rhythms and transcription factors binding were existed in the predicted promoter region. Phylogenetic analysis results suggested that MP was conserved in the leguminous plants. Results of tissue specific analysis revealed that expression level of GmMP was the lowest in leaves, the highest in SAM, it was speculated to participate in the auxin metabolic pathways.