miR-551b-3p在胰腺癌细胞和组织中的表达及其临床意义

Expression of miR-551b-3p in pancreatic cancer cells and tissues and its role in progression of pancreatic cancer

目的研究miR-551b-3p在胰腺癌细胞和组织中的表达,探讨其在胰腺癌发生发展中的临床意义。方法利用实时定量聚合酶链反应(RT-PCR)技术分别检测4种胰腺癌细胞和正常胰腺导管上皮细胞,以及76对胰腺癌组织和癌旁组织中miR-551b-3p的表达水平,并对miR-551b-3p的表达与胰腺癌患者临床病理学特征进行分析。结果 miR-551b-3p在PANC-1、Aspc-1、SW1990和Miapaca-2四种胰腺癌细胞中的相对表达量分别为(0.125±0.012)、(0.179±0.005)、(0.672±0.025)、(0.577±0.019),低于其在正常胰腺导管上皮细胞HPDE6C-7中的相对表达量,差异有统计学意义(P<0.05)。在胰腺癌组织和癌旁组织中miR-551b-3p的ΔCt值分别为(7.254±0.112)和(3.993±0.098),差异有统计学意义(P<0.001)。TNM分期Ⅰ、Ⅱ、Ⅲ和Ⅳ期胰腺癌组织中miR-551b-3p的ΔCt值分别为(4.343±0.032)、(5.325±0.112)、(6.987±0.098)和(9.132±0.212),差异有统计学意义(P<0.001)。有淋巴结转移和无淋巴结转移患者的胰腺癌组织中miR-551b-3p的ΔCt值分别为(8.492±0.021)和(6.676±0.103),差异有统计学意义(P=0.012)。高、中、低分化胰腺癌组织中miR-551b-3p的ΔCt值分别为(5.349±0.092)、(6.129±0.112)、(8.454±0.065),差异有统计学意义(P=0.002)。结论 miR-551b-3p的表达与胰腺癌的分化程度、淋巴结转移以及TNM分期相关。miR-551b-3p可能是提示胰腺癌临床进展的潜在标志物。

Objective This paper intends to analyze the correlation between the expression of miR-551b-3p and the clinicopathological characteristics in pancreatic cancer（PC）.Methods The expression of miR-551b-3p was analyzed by quantitative real-time PCR（RT-PCR）in pancreatic cancer cells,normal pancreatic ductal epithelial cells,and 76 paired PC samples.The relation between miR-551b-3p expression and clinicopathological characteristics was analyzed.Results The relative expression of miR-551b-3p in PC cells were lower than that in normal pancreatic ductal epithelial cells [（0.125±0.012）,（0.179±0.005）,（0.672±0.025）,（0.577±0.019）vs 1,P〈0.05].The relative expression of miR-551b-3p in PC tissues was also lower than that in peritumoral tissues [（7.254±0.112）vs（3.993±0.098）,P〈0.001].The relative expression of miR-551b-3p in tumors with different TNM stages were in stages Ⅰ,Ⅱ,Ⅲ,and Ⅳ,with a significant difference among them [（4.343±0.032）,（5.325±0.112）,（6.987±0.098）,（9.132±0.212）,P〈0.001].There was a significant difference between the relative expression of miR-551b-3p in PC with lymph node invasion and without lymph node invasion[（8.492±0.021）vs（6.676±0.103）,P=0.012].Different differentiation grades showed significantly different expression of miR-551b-3p[（5.349±0.092）,（6.129±0.112）,（8.454±0.065）,P=0.002].Conclusions The expression of miR-551b-3p is correlated with the differentiation grade,the TNM stage and the lymph node invasion,which may be a potential biomarker for the progression of PC.