柴胡三参胶囊对缺血性心律失常大鼠心肌细胞Cx43的影响

Effect of Chaihu Sanshen Capsule on Cx43 in Myocardial Cells of Rats with Ischemic Arrhythmias

目的探讨柴胡三参胶囊对缺血性心律失常大鼠心肌细胞间缝隙连接蛋白43(connexin 43,Cx43)表达和分布的影响,为柴胡三参胶囊的临床应用提供实验依据。方法大鼠随机分为空白组、假手术组、模型组、柴胡三参胶囊(青蒿组)、柴胡三参胶囊(常山组)、胺碘酮组、稳心颗粒组,每组各10只。相应干预10 d后,除空白组和对照组外,其余各组采取大鼠左冠状动脉前降支高位结扎法制作心肌缺血性心律失常模型,再用免疫组织化学S-P法检测Cx43蛋白在各组大鼠心肌中的表达水平及分布情况。结果柴胡三参胶囊(青蒿组)与柴胡三参胶囊(常山组)Cx43免疫组化灰度值比较差异无统计学意义(P>0.05);与模型组比较,柴胡三参胶囊心律失常评分降低(P<0.05)、死亡率降低、Cx43平均灰度值增高(P<0.05)及分布紊乱得到改善。结论柴胡三参胶囊(青蒿)与柴胡三参胶囊(常山)均能够改善心肌缺血时Cx43的表达及分布,保护心肌,从而减少缺血性心律失常的发生,且柴胡三参胶囊(青蒿)临床安全性更高。

Objective To investigate the effect of Chaihu Sanshen capsule on the expression and distribution of connexin 43 （Cx43） of gap junction in myocardial cells of rats with ischemic arrhythmias, and provide experimental basis for clinical application of Chaihu Sanshen capsule. Methods The rats were randomly divided into blank group, sham operation group, model group, Chaihu Sanshen capsule （Qinghao group）, Chaihu Sanshen capsule （Changshan group）, amiodarone group and Wenxin granules group, 10 rats in each group. After intervention for 10 d, in addition to the blank group and the control group, the other rats were made myocardial ischemic arrhythmias model of left anterior descending coronary artery ligation. The expression and distribution of Cx43 protein miocadial cells of rats was detected by immunohistochemical S-P method. Results The Cx43 gray scale of Chaihu Sanshen capsule （Qinghao group） and Chiahu Sanshen capsule （Changshan group） had no significant difference （P〉0.05）. The arrhythmia score of Chaihu Sanshen capsule was lower （P〈0.05） and a decrease in mortality, CX43 average gray value increased （P〈0.05） and distribution disorder to improve. Conclusion Chaihu Sanahen capsule （Qingbao） and Chaihu Sanahen capsule （Changshan） both can improve the expression and distribution of Cx43 in myocardial ischemia cell, protect cardiac muscle and to reduce the incidence of ischemic arrhythmias, and Chaihn Sanshen capsule （Qinghao） shows higher clinical safety.