苦参碱对胃癌抑制作用的实验研究

Anti-tumor effect of Matrine in the gastric cancer nude mice model

目的建立稳定的裸鼠胃癌模型,观察苦参碱的抗肿瘤作用。方法将20只胃癌模型裸鼠随机分为4组,分别为对照组、高浓度苦参碱(100 mg/kg·d^(-1),HM)组、低浓度苦参碱(50 mg/kg·d^(-1),LM)组、环磷酰胺(20 mg/kg·d^(-1),CTX)组。测定各组胃癌成瘤和区域淋巴结肿大情况,并采用病理学检查来明确有无癌肿浸润生长及区域淋巴结转移。结果建立裸鼠胃癌模型的成功率为100%。大体样本观察发现,HM组、LM组和CTX组胃癌成瘤肿物大小明显小于对照组;并无明显的区域淋巴结肿大;差异有统计学意义(P<0.05)。镜下观察发现,HM组无腹腔内区域淋巴结转移并无癌肿浸润生长,与CTX组无明显差异,但与对照组相比差异有显著性意义(P<0.05)。结论苦参碱有良好的抗肿瘤和抗转移活性;苦参碱对人胃癌潜在抑制作用提供理论基础。

Objective To observe the anti- tumor effect of Matrine in human gastric cancer nude mice model. Method Twenty gastric cancer nude mice models were randomized into four groups,high- dose Matrine（ 100 mg / kg·d^-1） group,low- dose Matrine（ 50 mg / kg·d^-1） group,cyclophosphamide（ CTX） group and control group. The tumor formation and lymphadenectasis were evaluated. The tumor cell infiltration growth and lymph node metastasis were examined in histopathological methods. Results Tumor formation were observed in all human gastric cancer nude mice models. Compared with control group,the high- dose,low- dose Matrine and CTX groups had a significantly less gastric tumor size and no obvious lymphadenectasis（ P〈0. 05）. And there was no tumor cell infiltration growth and lymph node metastasis in high-dose Matrine group. There was no significant difference in tumor size,tumor cell infiltration growth,and lymph node metastasis between CTX group and the high- dose Matrine group（ P〈0. 05）. Conclusion Matrine could play a role in gastric cancer treatment with it anti- tumor effect.