不同Ca^(2+)浓度养殖环境下三角帆蚌外套膜和内脏团组织钙调蛋白基因的表达

Expressions of Calmodulin Gene in Hyriopsis cumingii Mantle and Visceral Tissues Under the Culture Environment with Different Ca^(2+) Concentrations

为了探索钙调蛋白基因(Ca M)在淡水贝类珍珠形成中的作用,研究0、0.5、1.25、2和3 mmol/L 5个不同Ca^(2+)浓度水环境下养殖三角帆蚌,并在插核后的0、20、50、90和120 d对外套膜和内脏团组织采样,采用荧光定量方法检测组织Ca M表达量。结果表明:(1)在不同Ca^(2+)浓度中,Ca M基因外套膜和内脏团组织在0 mmol/L Ca^(2+)浓度下始终处于过低表达水平;0.5 mmol/L浓度下先降低后升高并维持稳定;1.25 mmol/L浓度下在20 d内脏团出现降低,之后升高至0 d水平并保持稳定;在2mmol/L浓度下表达量在20 d时显著上升达到各浓度中最高后保持高表达量状态;3 mmol/L浓度下在插核后20 d表达量升高,之后时期表达量开始不断降低(P<0.05)。(2)在插核后不同天数中,0 d各浓度表达差异不明显;之后各天数的表达出现明显差异。(3)相同条件养殖下,内脏团Ca M基因表达量高于外套膜。研究发现,0.5 mmol/L和2 mmol/L Ca^(2+)浓度会促进Ca M基因的表达,但0mmol/L和3 mmol/L的浓度则会抑制Ca M基因的表达。内脏团部位更有利于珍珠的形成。

In order to explore the role of calmodulin（Ca M）gene in the formation of freshwater mussel,Hyriopsis cumingii wascultured in water environment at 5 different Ca^2＋ concentrations of 0 mmol/L,0.5 mmol/L,1.25 mmol/L,2 mmol/L,and 3 mmol/L. At 0d,20 d,50 d,90 d,and 120 d after nucleus inserted,mantle and visceral were sampled,then the Ca M expressions in the tissues weredetected using fluorescent quantitative PCR. The results showed that ：1）The expression level of Ca M gene in mantle and visceral mass werealways low at 0 mmol/L Ca^2＋ concentration,decreased and then increased and remained stable at 0.5 mmol/L,decreased at the 20 d in visceralmass,then rose to level at 0 d and remain stable at 1.25 mmol/L,significantly increased at 20 d,reached the highest which was maintainedat 2 mmol/L among all 5concentrations,increased at 20 d after nucleus inserted,and decreased in the later period（P〈0.05）at 3 mmol/L. 2）There was no obvious difference among different concentrations at 0 d,and the expressions differentiated along different days after 0d. 3）Under the same culture conditions,the expression of Ca M gene in visceral mass was higher than that in mantle. It was found that theconcentration of 0.5 mmol/L and 2 mmol/L promoted the expression of Ca M gene,but the concentration of 0 mmol/L and 3 mmol/L inhibited theexpression of Ca M gene. Visceral mass was more conducive to the formation of pearl.