溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响

Effects of Lysophosphatidic Acid on the Migrations of Hepatoma Cells with Different Metastasis

旨在研究溶血磷脂酸(Lysophosphatidic acid,LPA)对两种转移性不同的肝癌细胞MHCC97H和Hep G2迁移行为的影响。采用Transwell法检测肝癌细胞的迁移能力,RT-PCR检测肝癌细胞中LPA受体(LPA receptor,LPAR)m RNA水平的表达。结果显示,LPA对低转移性肝癌细胞Hep G2的迁移没有明显影响,但显著促进高转移性肝癌细胞MHCC97H的迁移能力。LPA对两种细胞的增殖都没有明显影响。RT-PCR实验发现两种肝癌细胞中LPAR的表达呈现差异,LPAR1在MHCC97H细胞中表达,但Hep G2细胞不表达。用LPAR1/3抑制剂Ki16425阻断MHCC97H中LPAR1的作用后发现,LPA对MHCC97H细胞的促迁移作用消失,表明LPA通过与LPAR1作用促进了MHCC97H细胞的迁移。LPA对不同转移性肝癌细胞迁移能力的影响存在差异,该差异可能来自于细胞表达LPAR的不同。

The aim of this study is to investigate the effect of lysophosphatidic acid（LPA）on cell migration of two kinds ofhepatocellular carcinoma cells（MHCC97H and Hep G2）with different metastasis,and to evaluate the underlying mechanism. Transwellassay was employed to investigate the impact of LPA on the migration of the hepatocellular carcinoma cells,and RT-PCR to detect the m RNAexpression of LPA receptor（LPAR）in the hepatocellular carcinoma cells. Results showed that the LPA presented no obvious effect on themigration of Hep G2 cells in low metastasis,but significantly promoted the migration of MHCC97 H cells in high metastasis. LPA causedinsignificant impact to the proliferation of both cells. RT-PCR demonstrated that expressions of LPAR at m RNA level in MHCC97 H and Hep G2 cells were different,LPAR1 expressed in MHCC97 H cells,however,not in Hep G2 cells. Importantly,the promotion effect of LPA on themigration of MHCC97 H cells disappeared after Ki16425（a specific inhibitor of LPAR1/3）was used to block the role of LPAR1 in MHCC97 Hcells. This indicated that LPA promoted MHCC97 H cell migration via interaction with LPAR1. The effects of LPA on the migration of hepatomacells in different metastasis varied,which resulted from the different expressions of LPAR in these two cell types.