甘肃高山细毛羊DRB1基因内含子2微卫星多态性与生长速度的关联分析

Correlation analysis of DRB1 gene intron 2 microsatellite polymorphism and growth rate on Gansu Alpine Merino

通过220只甘肃高山细毛羊生长性能测定、DNA测序验证、微卫星DNA多态性检测和相关性分析,研究了甘肃高山细毛羊DRB1基因内含子2微卫星多态性与生长性能的相关性。结果表明,甘肃高山细毛羊DRB1基因内含子2微卫星重复单位是由GT/GA组成的复合型微卫星座位。研究共检测到13个等位基因和40种基因型,等位基因201、195和193 bp是优势等位基因,201/201 bp是优势基因型。该微卫星座位多态信息含量为0.830,杂合度为0.847,属高度多态座位。相关性分析表明,183、191、207和197 bp等位基因对应的甘肃高山细毛羊群体出生体质量极显著高于165、219、195、213和203 bp等位基因群体(P<0.01),同时显著高于179、189、193和201 bp等位基因群体(P<0.05),含有179、189、193和201 bp等位基因的群体的出生重显著高于165、219、195、213和203 bp等位基因的群体(P<0.05)。就甘肃高山细毛羊1月龄、2月龄、3月龄和6月龄体质量综合考虑,含有197和207 bp等位基因群体的体质量较高,从甘肃高山细毛羊DRB1基因内含子2微卫星座位选择考虑,在选种时尽量保留含有197和207 bp等位基因的个体作为种用,将可能提高甘肃高山细毛羊的出生体质量和出栏体质量(6月龄体质量)。

The correlation of the Gansu Alpine Merino DRB1 gene intron 2 microsatellite polymorphism and growth performance were analyzed by growth performance measurement,DNA sequencing and microsatellite DNA polymorphism detection. The results showed that the Gansu Alpine Merino DRB1 gene intron 2 microsatellite repeat unit was composed of GT and GA,which belonged to compound microsatellite type. Thirteen alleles and forty genotypes were detected on 220 Gansu Alpine Merino,allele 201,195 and 193 bp were advantagous,genotype 201/201 bp was advantagous. The microsatellite locus PIC was 0. 830,the heterozygosity was 0. 847,which was a highly polymorphic locus. Analysis of correlation indicated that the birth weights of 183,191,207 and 197 bp alleles on Gansu Alpine Merino groups were significantly higher than those of the 165,219,195,213 and 203 bp allele populations（P〈0. 01）,at the same time significantly higher than that of the 179,189,193 and 201 bp allele populations（P〈0. 05）,birth weights of the group containing 179,189,193 and 201 bp allele were significantly higher those that of the 165,219,165,213 and 203 bp alleles population（P〈0. 05）. For Gansu Alpine Merino,based on the comprehensive consideration of live weight of 1 month,2 months,3 months and 6 months of age,it was believed that the groups containing 197 and 207 bp allele had higher weights,from the perspective of Gansu Alpine Merino DRB1 gene intron 2 microsatellite locus selection,the individuals containing 197 and 207 bp allele should be reserved when selected as breeder,and it would be likely to improve the birth weight and slaughter weight（6 months） of Gansu Alpine Merino.