结缔组织生长因子rs9399005单核苷酸多态性与冠心病的关系

Relation Between Connective Tissue Growth Factor rs9399005 Gene Single Nucleotide Polymorphism and Coronary Heart Disease

目的探讨结缔组织生长因子(CTGF)rs9399005基因多态性与血清CTGF水平及冠心病的相关性。方法纳入冠心病组214例和正常对照组64例,酶联免疫吸附法检测其血清CTGF水平。采用Sanger法分析CTGF基因rs9399005单核苷酸多态性(SNP)。比较两组基线临床资料、血清CTGF和基因型分布频率。非条件Logistic回归分析CTGF rs9399005 SNP与冠心病的遗传易感性,比较不同基因型CTGF水平。分析CTGF水平与冠状动脉病变支数、冠心病严重程度的关系。结果两组年龄、吸烟、高血压、体质指数、高密度脂蛋白胆固醇、载脂蛋白A1、CTGF差异有统计学意义(P<0.05)。rs9399005基因型CC、CT、TT及等位基因C和T在两组间的分布有统计学差异(χ2值分别为12.935和17.148,均P<0.01)。携带CT发生冠心病的危险性是CC的1.134倍,TT是CC的1.406倍,T等位基因是C等位基因的1.327倍。血清CTGF水平在各基因型间差异有统计学意义(F=3.284,P=0.034)。各基因型间冠心病所累及的冠状动脉主要分支的差异有统计学意义(χ2=13.872,P=0.022)。结论CTGF rs9399005 SNP与冠心病的遗传易感性相关;等位基因T与冠心病严重程度、冠状动脉病变支数及血清CTGF水平相关。

Aim To investigate the relationship between connective tissue growth factor（CTGF） rs9399005 gene polymorphism and serum CTGF level,coronary heart disease（CHD）.Methods The serum CTGF levels were detected by enzyme linked immunosorbent assay in 214 cases of CHD and 64 cases of normal control group.CTGF gene rs9399005 single nucleotide polymorphism（SNP） was analyzed by Sanger method.Baseline clinical data,serum CTGF and genotype distribution frequencies were compared between the two groups.CTGF rs9399005 SNP and genetic susceptibility to CHD were analyzed by non conditional Logistic regression,and CTGF levels of different genotypes were compared.The relationships between CTGF level and coronary artery lesions,CHD severity were analyzed.Results The age,smoking,hypertension,body mass index,high density lipoprotein cholesterol,apolipoprotein A1,CTGF difference were statistically significant between the two groups（P 0.05）.The distribution of rs9399005 genotype CC,CT,TT,and allele C,T was statistically significant in the two groups（χ2was 12.935,17.148 respectively,all P 0.01）.The CHD risk of carrying CT was 1.134 times of CC,TT was 1.406 times of CC,T allele was 1.327 times of C allele.There was significant difference in serum CTGF level between different genotypes（F = 3.284,P = 0.034）.There was significant difference in the main branche lesions of CHD among all genotypes（χ2= 13.872,P = 0.022）.Conclusions CTGF rs9399005 SNP is related to genetic susceptibility of CHD.T alleles is associated with the severity of CHD,coronary artery lesions and serum CTGF level.