AB血清对人体外终末期红系分化进程的影响

Effect of AB serum on human terminal erythroid differentiation ex vivo

目的:探究体外诱导造血干细胞向红系分化过程中AB血清对终末期红系分化进程的影响。方法:从脐带血中用免疫磁珠法分离提取CD34+细胞,体外诱导其向红系分化。根据培养体系中是否添加AB血清,设置实验组为添加AB血清组,对照组为不添加AB血清组。通过检测细胞表面红系分化标志分子糖化血红蛋白A(glycophorin A,GPA)、人红细胞阴离子交换蛋白Band3及整合素α4(α4-integrin)的表达反映终末红系分化进程,用流式细胞术检测红系细胞终末分化脱核情况。结果:与对照组相比,实验组GPA阳性细胞百分率较高,Band3表达较高,而α4-integrin表达较低(P<0.05)。实验组细胞脱核百分率明显高于对照组(P<0.05)。结论:在体外红系发育培养体系中,AB血清对终末期红系分化进程有一定的促分化、促脱核作用。

Objective： To demonstrate the effect of AB serum on terminal erythroid differentiation ex vivo. Methods. After separation of CD34＋ cells from cord blood, the cells were cultured and divided into a control group and an experimental group. The effects of AB serum were examined by the expressions of different markers （GPA, Band3 and α4-integrin） for erythroblast differentiation and enudeation by flow cytometry.Results： The CD34＋ cells were successfully differentiated to enudeated red blood ceils. There were evident differences among the expressions of GPA, Band3 and a4-integrin between the 2 groups. The percentage of GPA positive cells in the experimental group was bigger than that in the control group in every time point. The expression of Band3 in the experimental group was higher than that in the control group. The expression of a4-integrin in the experimental group was lower than that in the control group. In addition, the enucleation rate in the experimental group was higher than that in the control group. Conclusion： AB serum can promote the cell differentiation and enucleation during terminal erythroid differentiation in vitro. hematopoietic stem cell; terminal erythroid differentiation; AB serum