摘要
目的:探讨长链非编码RNA GAS5在胃腺癌组织中的表达及其对胃腺癌SGC-7901细胞生长的影响。方法:筛选2012年1月至2014年12月间于我院普外科行手术切除的胃腺癌(gastric adenocarcinoma)患者的肿瘤及对应癌旁组织40例,运用qRT-PCR技术检测lncRNA-GAS5在组织中的表达水平,分析lncRNAGAS5表达与患者临床病例资料间的相关性;通过在SGC-7901细胞中过表达lncRNA-GAS5,采用MTT试验检测过表达lncRNA-GAS5后SGC-7901细胞增殖变化,流式细胞仪检测细胞凋亡变化,qRT-PCR及Western-blot检测PTEN表达变化。结果 :lncRNA-GAS5在胃腺癌组织中表达水平显著低于对应癌旁组织(t=3.773,P<0.001),胃癌组织中lncRNA-GAS5低表达与肿瘤直径增大(P=0.035)及高TNM分期(P=0.031)显著相关;过表达lncRNA-GAS5后可显著抑制细胞增殖(P<0.05)并上调凋亡细胞比例(P=0.039);qRT-PCR及Western-blot结果证实,过表达lncRNA-GAS5后可显著促进PTEN m RNA及蛋白表达水平(P<0.001)。结论 :lncRNA-GAS5在胃腺癌组织中表达下调并与肿瘤恶性临床病理特征有关,lncRNA-GAS5可能通过促进抑癌基因PTEN的表达来抑制胃腺癌生长。
Objective To study the expression of long noncoding RNA GAS5 （lncRNA-GASS） in gastric adenocarcinoma and its role for regulating cell proliferation and apoptosis. Methods Gastric adenocareinoma and matched tumor adjacent tissues from 40 patients underwent surgery were collected in General Surgery Department of the hospital between January, 2012 and December in 2014. The expression of lncRNA-GAS5 was detected by qRT-PCR. LneRNA-GAS5 specific plasmid was transfected into SGC-7901 cells. MTr assay and flow cytometer （FCM） were used to measure cell proliferation and apoptosis. The expression of PTEN was detected by qRT-PCR and western-blot. Results Expression of lncRNA-GAS5 in gastric adenocarcinoma tissues was significantly lower than that in para-gastric adenoearcinoma tissues （t = 3.773, P 〈 0.001）. Low expression of lncRNA-GAS5 was positively associated with large tumor diameter （P = 0.035） and advanced TNM stage （P = 0.031）. Over expression of lncRNA-GAS5 can suppress cell proliferation （P 〈 0.05）, and up-regulate cell apoptosis ratio （P = 0.039）. It can be testified by qRT-PCR and western-blot that over expression of lncRNA-GAS5 can be down-regulated in gastric adenocarcinoma tissues, which is related with malignant tumor clinical pathology, and lncRNA-GAS5 can inhibit adenocarcinoma tissues growth by promoting PTEN.
出处
《实用医学杂志》
CAS
北大核心
2016年第24期4005-4008,共4页
The Journal of Practical Medicine
