Wip1、Shh、Gli1蛋白在皮肤恶性黑色素瘤细胞中的表达及临床意义

Expression and clinical significance of Wip1,Shh and Gli1 protein in cutaneous malignant melanoma

目的:探讨Wip1、Shh、Gli1蛋白在皮肤恶性黑色素瘤(CMM)中的表达及其临床意义。方法:免疫组化法检测Wip1、Shh、Gli1蛋白在31例CMM与混合痣中的表达,分析各蛋白表达之间的关系,及其与CMM肿瘤临床分期、复发、转移的关系。结果 :Wip1、Shh、Gli1蛋白在CMM中的阳性表达率均显著高于混合痣组(P<0.05)。在CMM中,Wip1、Gli1蛋白阳性表达率与肿瘤临床分期、复发、转移均呈正相关(P<0.05),Wip1蛋白与Gli1蛋白阳性表达率呈正相关(P<0.01);Shh蛋白阳性表达率与肿瘤转移呈正相关(P<0.05),与Gli1蛋白表达无明显相关。结论:Wip1、Shh、Gli1蛋白在CMM的发生及发展中可能发挥重要作用。Wip1、Gli1蛋白表达可以作为CMM预后的评价指标。Gli1蛋白高表达主要受Wip1蛋白的调节,为信号通路靶向阻断剂在CMM治疗中提供指导意义。

Objective To investigate the expression and clinical significance of Wipl, Shh and Glil protein in the cutaneous malignant melanoma （CMM）. Methods Immunohistoehemistry EnVision method was used to detect the expression of Wipl, Shh and Glil protein in compound nevus group and CMM group, and the statistical methods was applied to analyses the relationship between the expression and tumor clinical stage,tumor recurrence and metastasis and the correlation among the proteins. Results The positive rates of Wipl, Shh and Glil protein in CMM group were significantly higher than those in compound nevus group （P 〈 0.05）. In CMM group, the significant correlation between the expression of Wipl ,Glil and tumor clinical stage, tumor recurrence and metastasis was observed （P 〈 0.05）. Positive correlation was seen between the expression of Wipl and Glil （P 〈 0.01 ） and the significant correlation between the Shh positive expression and the tumor metastasis was also observed （P = 0.02）. The coefficient between Shh and Glil had no statistical significance. Conclusions Wipl and Glil protein might play an important role in the development and occurrence of CMM and can be regarded as an assessment index of the prognosis of CMM. Glil is mainly regulated by Wipl in CMM and the result can provide guidance for the signaling pathway blockers in CMM treatment.