磷酸甘油酸变位酶1在生精功能障碍睾丸组织中的表达及意义

Expression and significance of PGAM1 in testicular tissue of spermatogenesis dysfunction

目的:检测磷酸甘油酸变位酶1(PGAM1)在不同程度生精功能不良睾丸组织中的表达情况,并分析PGAM1表达与临床病理参数的关系。方法:收集生精功能正常、轻度生精功能不良、重度生精功能不良、唯支持细胞综合征患者睾丸组织标本,统计性激素六项结果,采用免疫组化、qRT-PCR检测PGAM1在不同程度生精功能不良睾丸组织中的表达情况。结果:免疫组化结果显示PGAM1的高表达率在生精功能正常、轻度生精功能不良、重度生精功能不良及唯支持细胞综合征患者各组间比较,差异具有统计学意义(P=0.000),PGAM1表达与患者的年龄、睾丸体积、LH及T表达均无明显相关(P>0.05);而PGAM1低表达患者FSH水平高于PGAM1高表达患者(P=0.002 3);qRT-PCR结果提示PGAM1 mRNA在生精功能正常及唯支持细胞综合征患者睾丸组织中表达水平明显高于重度生精不良睾丸组织中的表达,差异具有统计学意义(P=0.000),PGAM1 mRNA表达与患者的年龄、睾丸体积、LH及T表达均无明显相关(P>0.05),而在FSH≤12 mIU/mL患者睾丸组织中的表达明显高于FSH>12 mIU/mL患者(P=0.043 0)。结论:PGAM1的表达与睾丸生精功能障碍有关,其低表达与重度生精功能不良的形成相关,其异常高表达与唯支持细胞综合征的形成有关。

Objective To detect the expression of PGAM1 in testicular tissue of different degree of spermatogenesis dysfunction, and to analyze the relationship between PGAM1 expression and clinical pathological parameters. Method The testicular tissue samples were collected from the patients with normal spermatogenesis, mild and severe spermatogenesis dysfunction and sertoli-cell-only syndrome respectivley.The clinical pathological parameters including sex hormone and seminal plasma biochemical test results were observed.The expression of PGAM1 was detected by immunohistochemistry and qRT-PCR in the testis of different degree of spermatogenesis dysfunction. Results Tile immunohistochemistry results showed that the intra-comparison of high expression rate of PGAM1 in patients with normal spermatogenesis, spermatogenic dysfunction, mild and severe spermatogenic dysfunction and sertoli-cell-only syndrome indicated statistical difference （P = 0.000）. The expression of PGAM1 was not significantly related with patient＇s age, testicular volume and the expression of LH and T（P 〉 0.05）. The FSH level in patients with low PGAMI expression was higher than that in patients with high expression of PGAM1 and the differencebetween the groups was statistically significant （P = 0.0023）. The results of qRT-PCR showed that the expression of PGAMlmRNA in testicular tissue of patients with normal spermatogenesis and those with sertoli- cell-only syndrome was significantly higher than that in testicular tissue of patients with severe spermatogenesis dysfunction and the difference was statistically significant （P = 0.000）. The expression of PGAMlmRNA and patient＇s age, testicular volume, LH and T expression were not significantly related （P 〉 0.05）, while the expression in testis tissues of patients with FSH ≤ 12 mIU/mL was significantly higher than that in testis tissues of patients with FSH〉 12 mlU/mL（P = 0.0430）. Conclusions The expression of PGAM1 is related to the dysfunction of testicularspermatogenesis. Its low expression is related to the formation of severe dysfunction of spermatogenesis, and its abnormal high expression is related to the formation ofsertoli-cell-only syndrome.