桑酮碱改善2型糖尿病大鼠的糖脂代谢

Sangtongjian Mixture ameliorates glucose and lipid metabolism in type 2 diabetic rats

目的观察桑酮碱对2型糖尿病大鼠糖脂代谢、胰岛素抵抗、脂肪细胞因子的影响及其作用机制。方法 140只大鼠喂以高脂饲料联合链脲佐菌素(STZ)建立2型糖尿病模型,空腹血糖值>16.7 mmol/L,大鼠随机分为模型组、二甲双胍(180 mg/kg)组、桑酮碱(73.5、147、294 mg/kg)组,空白组10只,治疗组灌胃给药12周。给药后每2周测定大鼠空腹血糖(FBG);给药12周后,测定糖化血清蛋白(GSP)、糖化血红蛋白(GHb)及血脂指标(TC、TG、HDL-C、LDL-C);放免法测定血清胰岛素水平,并计算胰岛素抵抗指数(HOMR-IR)和胰岛素敏感指数(ISI);酶联免疫吸附实验(ELISA)测定血清脂联素和瘦素水平。结果桑酮碱显著降低2型糖尿病大鼠的空腹血糖、GSP、GHb、TC、TG、LDL-C、瘦素水平和胰岛素抵抗指数,升高HDL-C、脂联素水平和胰岛素敏感指数。结论桑酮碱能改善2型糖尿病大鼠糖脂代谢,是通过改善胰岛素抵抗和调节脂肪细胞因子水平而成的。

AIM To observe the effects of Sangtongjian Mixture（ STJ） on glucose and lipid metabolism,insulin resistance and fat cytokines in type 2 diabetic rats,and their mechanisms of action. METHODS One hundred and forty rats fed on the combination of STZ and high fat diet were established as the type 2 diabetic models.Fasting blood glucose（ FBG） level reached more than 16. 7 mmol / L and then the rats were randomly divided into model group,metformin（ 180 mg / kg） group,STJ（ 73. 5,147 and 294 mg / kg） groups. Ten rats were set as the blank group. Each treatment group was intragastrically given the corresponding agents for twelve weeks. The fasting blood glucose levels of rats were measured once every two weeks after the administration. After a 12-week administration period,glycosylated serum protein（ GSP）,glycosylated hemoglobin（ GHb） and lipid profile indices（ TC,TG,HDL-C and LDL-C） were determined. The serum insulin level was measured by radioimmunoassay,and homeostasis model assessment of insulin resistance（ HOMA-IR） and insulin sensitivity index（ ISI） were calculated. The levels of serum adiponectin and leptin were detected by ELISA. RESULTS STJ remarkably decreased the levels of FBG,GSP,GHb,TC,TG,LDL-C,leptin and HOMR-IR in type 2 diabetic rats. Furthermore,STJ also significantly increased the levels of HDL-C,adiponectin and ISI. CONCLUSION STJ can improve glucose and lipid metabolism in type 2 diabetic rats by ameliorating insulin resistance and regulating fat cytokine levels.