基于核磁共振的寻常型银屑病患者血浆代谢组学研究

An 1 HNMR-based plasma metabonomic study of patients with psoriasis vulgaris

目的通过基于核磁共振氢谱(nuclear magnetic resonance 1 H spectroscopy,1 HNMR)的代谢组学技术寻找寻常型银屑病患者血浆潜在生物标记物,探讨其相关代谢通路并进行分析。方法应用1 HNMR技术对10例寻常型银屑病患者和12例健康志愿者血浆进行检测,对所得数据分别采用主成分分析法(principal component analysis,PCA)和正交偏最小二乘法判别分析法(orthogonal partial least squares discriminant analysis,OPLS-DA)进行模式识别分析,寻找寻常型银屑病患者与健康者血浆差异性代谢物,根据差异性代谢物的变化构建相关代谢通路图并进行分析。结果模式识别分析具有较好的区分度和预测性,寻常型银屑病患者血浆中极低密度脂蛋白、低密度脂蛋白、脂类、亮氨酸、异亮氨酸、缬氨酸、β-羟基丁酸、乳酸、丙氨酸、精氨酸、N-乙酰糖蛋白、乙酰乙酸、谷氨酸、丙酮酸、谷氨酰胺、柠檬酸、肌酸、肌酐、葡萄糖、天冬氨酸和苯丙氨酸含量明显增高。结论寻常型银屑病患者不仅存在脂类代谢、糖代谢和氨基酸代谢的紊乱,还伴随着炎症反应发生和肝肾功能损害。该结果加深了对寻常型银屑病发病机制的认识,为疾病的诊断和治疗提供了新思路和新方法。

Objective To search for the potential plasma biomarkers for patients with psoriasis vulgaris by metabonomics technology based on nuclear magnetic resonance 1H spectroscopy （1HNMR）, and to explore the related metabolic pathway. Methods 1HNMR technique was applied to examine the plasma samples from 10 patients with psoriasis vulgaris and 12 healthy volunteers. The data were analyzed for pattern recognition using principal component analysis （PCA） and orthogonal partial least squares discriminant analysis （OPLS-DA）. The differential metabolites in plasma between patients psoriasis vulgaris and healthy volunteers were analyzed, and the metabolic pathways were constructed according to the changes of the differential metabolites. Results Pattern recognition analysis had a satisfactory discriminating and predicting performance. Compared with healthy volunteers, the levels of very low density lipoprotein （VLDL）, low density lipoprotein （LDL）, lipids, leucine, isoleucine, valine, β-hydroxybutyrate, lactate, alanine, arginine, N-acetylglycoproteins, acetoacetate, glutamate, pyruvate, glutamine, citrate, creatine, creatinine, glucose, aspartate and phenylalanine in plasma of patients with psoriasis vulgaris were greatly increased. Conclusion Our results suggest that patients with psoriasis vulgaris have disorders of lipid metabolism, glucose metabolism and amino acid metabolism, accompanied by inflammation and functional damage to the liver and kidney, which may improve our understanding of the pathogenesis of psoriasis vulgaris.