在烟草中瞬时表达迷迭香酸的研究

Transient Expression of Rosmarinic Acid in Tobacco

遗传转化一直以来都是研究药用植物次生代谢物调控的重要手段,虽然具有重现性好的优势,但获得稳定转化的毛状根体系或转基因植株往往费时费力,该方法无法满足大批量药用植物次生代谢调控相关基因的研究需求。农杆菌介导的瞬时转化以其易操作、低成本、短周期的优势,已被广泛应用于植物功能基因的研究中。然而目前药用植物的功能基因研究还常使用稳定遗传转化,或一些高成本、高难度的瞬时转化方法(如基因枪、原生质体转化等)。因此,本研究利用pEAQ载体在本氏烟草叶片中高效表达了一个或多个外源基因,这将为外源基因在烟草中快速有效的表达提供新途径,也为进一步探究其功能提供了一条思路。多个外源基因的高效共表达也为在本氏烟草中异源构建次生代谢途径提供了可能。

Genetic transformation is an important mean in the study of secondary metabolism regulation of medicinal plants. The advantage of this method is its high reproducibility. However, it is time-consuming to get stable hairy root system or difficult for transgenic plants,and it can not operate in large scale as well. Transient transformation mediated by Agrobacterium is easy to operate with low cost and short period, which is widely used in the study of functional genes. At present, stable genetic transformation and some high cost,difficult transient transformation methods （e. g. gene gun, protoplast transformation） are usually used in the study of the function genes in medicinal plants. In this paper, one or more exogenous genes were expressed efficiently in Nicotiana benthamiana leaf by using the pEAQ system. The results would provide a new way for the exogenous genes express efficiently in tobacco,and also provide an idea way for further study of the genes function. Meanwhile, the high-efficient co-expression of exogenous genes would also provide the possibility for the construction of secondary metabolic pathways in N. benthamiana.