土壤来源链霉菌KIB-717抗植物病原真菌活性成分研究

Secondary Metabolites from a Soil-derived Streptomyces sp.KIB-717 and Their Inhibitory Activity on Plant Pathogenic fungi

运用中压MCI柱色谱和半制备HPLC等分离手段对云南省腾冲高黎贡山土壤来源链霉菌KIB-717次级代谢产物进行分离纯化,得到3个单体化合物。运用NMR和MS等波谱方法,并参考相关文献,对所得化合物进行结构分析,分别鉴定为β-rubromycin(1)、γ-rubromycin(2)、griseorhodin F(3)。运用滤纸片法测定这三个化合物对植物病原真菌的抑菌活性。抑菌实验首次证明化合物1和2对番茄灰霉病菌(Botrytis cinerea)、稻瘟病病菌[Magnaporthe grisea(Hebert)Barr.]等所测植物病原真菌表现出一定的抑制作用。

Three compounds, β-rubromycin （1）,γ-rubromycin （2） and griseorhodin F （3）,were separated and purified by medium-pressure MCI column and semipreparative HPLC from the fermentation broth of Streptomyces sp.KIB-717,which was isolated from soil samples collected in Gaoligongshan in Tengchong from Yunnan Province,China.The structures of three isolates were characterized through extensive analysis of NMR and mass spectrometry data and by comparison the NMR data with that reported in literatures.The antifungal experiments demonstrated that compounds 1 and 2 possessed significant antifungal activity against most of the tested plant pathogenic fungi,such as Botrytis cinerea and Magnaporthe grisea （Hebert） Barr.