HPLC同时测定地黄中5种苷类成分的含量

Simultaneous Determination of Five Glycosides in Rehmanniae Radix by HPLC

建立高效液相色谱法同时测定地黄中梓醇、地黄苷D、益母草苷、毛蕊花糖苷和异毛蕊花糖苷含量的方法。采用Synergi^(TM)Hydro-RP 80色谱柱(250 mm×4.6 mm,4μm);流动相为乙腈-0.2%磷酸水,梯度洗脱;流速为1 m L/min,柱温35℃;检测波长:梓醇、地黄苷D和益母草苷为203 nm;毛蕊花糖苷和异毛蕊花糖苷为334 nm。地黄中5种苷类成分分离良好,各成分含量与峰面积在测定范围内均呈良好的线性关系(r≥0.9990),加样回收率分别为96.43%、97.52%、96.16%、100.48%、97.21%,RSD分别为1.44%、2.56%、1.07%、1.98%、3.35%。该方法简单、快捷,重现性好,准确率高,可为地黄的质量控制研究提供依据。

To establish an HPLC method for simultaneous determination of catalpol,rehmannioside D,leonuride,acteoside,isoacteoside in Rehmanniae Radix.SynergiTM Hydro-RP 80 column （250 mm× 4.6 mm,4 μm）was used,with acetonitrile-0.2% phosphoric acid as the mobile phase in gradient elution mode.The column temperature was 35 ℃,with flow rate1.0 mL/min.The UV detection wavelength was set at the maximum absorption wavelength,203 nm for catalpol,rehmannioside D and leonuride,334 nm for acteoside and isoacteoside.The five kinds of glycosides in Rehmanniae Radix were separated well,and the linear relation was obtained（r≥0.9990）.The average recoveries were 96.43%,97.52%,96.16%,100.48%and 97.21%,with RSD 1.44%, 2.56%,1.07%,1.98%,3.35%,respectively.Themethod is simple,accurate,reproductive,and can provide the basis for the quality control of Rehmanniae Radix.