菊芋源菊粉对肥胖小鼠缓解作用的研究

Mitigation Effect of Jerusalem artichoke Inulin on Obese Mice

通过采用高脂饲料诱导建立小鼠肥胖模型,对模型小鼠分别采用9.0‰生理盐水(模型对照组)、低[2.5g/(kg·d)]、高[10 g/(kg·d)]2种剂量的菊粉(低剂量菊粉组、高剂量菊粉组)和药物奥利司他(奥利司他组)处理,同时用普通饲料喂养、9.0‰生理盐水处理的小鼠作为空白对照,通过测定小鼠的体重、肝体比和血清学指标(总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇),并观察肝脏油红O染色切片,研究菊粉对肥胖小鼠的缓解作用。结果显示,与模型对照相比,菊粉处理能够降低小鼠的体重,分别降低了9.03%和7.77%,总胆固醇含量下降7.91%和12.02%,高密度脂蛋白胆固醇与低密度脂蛋白胆固醇的比值提高16.39%和73.51%,肝体比降低13.81%和44.20%,切片结果显示小鼠肝脏的脂肪滴数量较模型对照组少。本研究结果表明菊粉可以降低血清中的总胆固醇含量,提高高、低密度脂蛋白胆固醇之间的比值,减少肝脏的脂肪堆积,降低肝体比,对小鼠肥胖具有缓解作用。

In order to investigate the mitigation effect of Jerusalem artichoke inulin on obese mice,the obesity mice model was established by using high-fat feed.Forty model mice were randomly and equally divided into model group,low inulin dose group,high inulin dose group and orlistat group to receive normal saline,low dose of inulin,high dose of inulin and orlistat,respectively,by gavage.Meanwhile,ten mice were selected as blank control group,which received fundamental feed and normal saline.After four weeks,the changes in body weight,hepatopancreas index,triglyceride （TG）,total cholesterol （TC）,high and low density lipoprotein cholesterol （HDL-C and LDL-C） were evaluated and the liver oil red O staining were compared between the four groups.The results were as follows：Compared with model group,inulin reduced mice weight （lowered 9.03% and 7.77%）,total cholesterol （lowered 7.91% and 12.02%）,HDL-C/LDL-C （lowered 16.39% and 73.51%） ,hepatopancreas index （lowered 13.81% and 44.20%）.The liver slices showed that the number of fat droplets under inulin treatments was less than that of model group.These results indicated that inulin had the mitigation effect on obesity through decreasing total cholesterol,hepatopancreas index and fat droplets as well as increasing HDL-C/LDL-C.