7个黑木耳菌株遗传差异性分析

Study on Genetic Polymorphism of 7 Auricularia auricula Strains

采用拮抗试验、酯酶同工酶和分子标记对引进7个黑龙江主栽黑木耳菌株进行遗传差异分析。结果表明,在黑木耳遗传差异性分析中,3种方法具有一致性。通过酯酶同工酶与分子标记聚类分析,均能将来自于黑龙江同一地域的黑木耳分为4个类别:黑威9号与981遗传距离最近为第一类,黑威10号、8808与第一类2个菌株遗传距离较近为第二类,黑29与第一类、第二类4个菌株遗传距离较远为第三类,而9809、H916与以上5个菌株遗传距离最远为第四类。同时在拮抗试验中黑29、9809、H916三个遗传距离较远的菌株也表现出明显的拮抗现象。研究结果为黑木耳新品种引选以及种质资源评价分析提供技术支撑。

Genetic diversity of seven strains of A uricularia auricular from Heilongjiang province were analyzed by antagonistic reaction, esterase isoenzyme and DNA marker respectively. Both of the data from RAPD／ISSR and antagonistic effect indicated that seven strains could be derided into four distinct groups, such as the first group included Heiwei 9 and 981, the second group included Heiwei 10 and 8808, the third group included single strain Hei29, the 9809 and H916 were classified to the last group. Meanwhile, the antagonistic reaction showed that the three isolates with longer genetic distance of Hei 29, 9809 and H916 also showed obvious antagonistic effect. The present study indicated that results obtained by the three methods were consistent, which could provide technical support for new species selection and germplasm resources evaluation of A. auricula.