白蔹的化学成分研究

Study on the Constituents of Ampelopsis Japonica(Thunb.) Makino

目的研究中药白蔹的化学成分。方法采用大孔吸附树脂、硅胶柱色谱、聚酰胺色谱、葡聚糖凝胶(sephadex LH-20)柱色谱、十八烷基硅烷键合硅胶(ODS)色谱等手段进行分离纯化,根据化合物的理化性质和波谱学等方法鉴定中药白蔹所含化学成分的结构。结果从白蔹植物的70%乙醇提取物中分离得到9个化合物,分别鉴定为尿苷(uridine,1),腺苷(adenosine,2),阿魏酸(ferulic acid,3),原儿茶酸(protocatechuate,4),没食子酸(gallic acid,5),大黄素-8-O-β-D-吡喃葡萄糖苷(emodin-8-O-β-D-glucopyranoside,6),咖啡酸(caffeic acid,7),β-谷甾醇(β-sitosterol,8),7β-羟基-β-谷甾醇(7β-hydroxy-β-sitosterol,9)。结论化合物4和化合物9是从蛇葡萄属(Ampelopsis Michx.)植物中首次分离得到,化合物1~3和化合物7为首次从白蔹Ampelopsis japonica(Thunb.)Makino植物中分离得到。

Objective To study the chemical constituents of the Ampelopsis japonica（Thunb. ） Makino. Methods Separation and purification were carried out by macroporous adsorption resin, silica gel column chromatography, polyamide chromatography, Sephadex LH- 20 column chromatography, octadecyl silane bonded silica gel（ODS） chromatography. The physico-chemical properties and spectroscopic methods were used to identify the structure of Ampelopsis japonica（Thunb. ） Makino. Results 9 compounds were obtained from the 70% ethanol-water extracts of the Ampelopsis japonica（Thunb. ） Makino. The structures were determined as Uridine（1）, Adenosine（2）, Ferulic Acid（3）, protocatechuate（4）, gallic acid（5）, emodin - 8 - O - β - D - glucopyranoside（6）, Caffeic acid（7）, β - sitosterol（8） and 7 β-hydroxy- β-sitosterol（9） by comparison of their spectral data with the reported data, respectively. Conclusion s Compounds 4 and 9 were isolated from genus Ampelopsis for the first time, compound 1-3 and 7 were isolated from Ampelopsis japonica（Thunb. ） Maki- no for the first time.