VEGF165基因治疗鼠脑梗塞分子机制的初步研究

Preliminary of molecule mechanism of rat's infarct brain With VEGF165 gene therapy

目的 :探讨血管内皮生长因子 16 5 (VEGF16 5 )基因治疗鼠脑梗塞中的有关分子表达情况 ,为临床研究脑硬塞的分子机制提供依据。方法 :采用改进的线栓法制成Wistar大鼠永久性大脑中动脉闭塞 (MCAO)模型 ,将质粒载体与VEGF16 5 (VascularEndothelialGrowthFactor)基因构成的重组DNA经颅骨注入到梗塞区 ,7d后断头取脑 ,用免疫组化的方法显示脑中的HSP70 (HeatShockProtein)和VEGF蛋白表达情况。结果 :治疗组VEGF和HSP70表达显著增高。结论 :缺血脑组织具有摄取并表达外源性VEGF基因的能力 。

Objective To observe the expression of certain molecule in brain of MCAO rats in VEGF 165 gene therapy to provide some molecule evidence for clinical cerebral infarct research.Methods To establish a rat's permanent model of MCAO by nylon suture embolization, VEGF 165 gene was directly injected through skull into the ischemia area,after seven days, the rats were killed,immunohistochemistry was used to show the expression of HSP70 AND VEGF proteins in rats brain.Results There was a significant increase at level of HSP70 and VEGF's expression.Conclusion Ischemic brain tissue could take in and express the VEGF 165 gene, and VEGF 165 gene could induce HSP70 protein.