黑龙江省全沟硬蜱携带伯氏疏螺旋体广义种和斑点热群立克次体的研究

Study on Borrelia burgdorferi sensu lato and spotted fever group Rickettsia in Ixodes persulcatus in Heilongjiang Province

为了解黑龙江省牡丹江市、鹤岗市萝北县、哈尔滨市通河县全沟硬蜱携带伯氏疏螺旋体和斑点热群立克次体的情况,在获得25只全沟硬蜱细菌组学数据的基础上,通过PCR扩增16S r RNA和fla基因检测伯氏疏螺旋体,扩增glt A和r Omp A基因检测斑点热群立克次体。结果显示,伯氏疏螺旋体的阳性率为20.0%(5/25),斑点热群立克次体的阳性率为68.0%(17/25)。另外,伯氏疏螺旋体阳性样品均显示斑点热群立克次体阳性,这两种病原的混合感染率为20.0%(5/25)。序列分析结果显示,16S r RNA基因与伽氏疏螺旋体Nov405菌株的相似性为99.65%,fla基因与伽氏疏螺旋体SZ菌株的相似性为99.73%;glt A和r Omp A基因与塔拉塞维奇立克次体暂定株(Candidatus Rickettsia tarasevichiae)的相似性均为100%。结果表明,斑点热群立克次体(Candidatus Rickettsia tarasevichiae)和伯氏疏螺旋体广义种在全沟硬蜱中广泛存在,这两种病原的混合感染增加了蜱传人兽共患病的风险。

The aim of this study is to understand Borrelia burgdorferi sensu lato and spotted fever group Rickettsia infections in Ixodes persuicatus in Heilongjiang Province,China. Based on the bacterial genomics data of 25 I. persulcatus, PCR method was performed and detected B. burgdorferi sensu lato based on 16S rRNA and fla genes and spotted fever group Rickettsia based on gltAand rOmpA genes. In result,the positive rate of B. burgdorferi sensu latoand spotted fever group Rickettsia in I. persulcatus were 20.0% （5/25） and 68. 0%（17/25）,respectively. The co-infection rate was 20. 0%（5/25）.The sequences ofl6S rRNAand fla genes blasted with RgariniiNov 405 and SZ strains,with 99. 65%and 99. 73 %identity, respectively. The sequences of gltAandrOmpA genes were blastedwith Candidatus Rickettsia tarasevichiae,with 100% identity. In conclusion,the high prevalence of co-infection of two pathogens increased a risk of zoonosis in these regions.