miR-150—5P在肝细胞癌组织中的表达及其临床意义

The expression and clinical significance of miR-150-5P in patients with hepatocellular carcinoma

目的检测miR-150-5p在肝细胞癌组织及癌旁组织中的表达，分析miR-150-5p表达水平与肝细胞癌患者临床病理特征和术后5年总体生存率的关系。方法利用实时定量聚合酶链反应（qRT-PCR）和原位杂交方法检测91例肝细胞癌组织及配对癌旁组织中miR-150-5P的表达情况，并进行统计分析。结果qRT-PCR结果显示miR·150-5p在肝细胞癌组织中表达水平显著低于癌旁组织，原位杂交结果显示肝细胞癌组织中miR-150-5P阳性率显著低于癌旁组织（χ2=4．958，P=0．046）。χ2检验结果显示肝细胞癌组织中miR-150-5P的表达水平与肝细胞癌患者就诊时淋巴转移情况有关（χ2=8．617，P=0．006），而与其他临床病理特征之间无关。生存曲线显示miR-150-5P表达水平与肝细胞癌患者术后5年总体生存率密切相关（χ2=6．184，P=0．013）。结论miR-150-5P在肝细胞癌组织中表达降低，其表达与肝细胞癌患者淋巴转移情况有关，亦与肝细胞癌患者术后5年总生存率有关。

Objective To explore the expression and clinical significance of miR-150-5P in patients with hepatocellular carcinoma （HCC）. Methods Expressions of miR-150-SP were analyzed by quantita- tive real-time polymerase chain reaction （qRT-PCR） and in situ hybridization （ISH） in cancerous and ad- jacent tissues of 91 HCC patients. Statistical analysis was conducted by SPSS 22. 0 software. Results qRT-PCR revealed that the mRNA expression level of miR-150-5P was significantly lower in HCC tissues than adjacent tissues. ISH revealed that the positive rate of miR-150-5P was downregnlated in HCC tissues compared to adjacent tissues（χ2 =4. 958, P =0. 046）. Chi-square analysis revealed that high miR-150-5P expression was inversely associated with lymph node status of HCC patients, which was statistically significant （χ2 = 8.617,P = 0. 006 ）. Kaplan-Meier curves revealed that high miR-150-5P expression was positivelycorrelated with 5-year overall survival rates of HCC patients after surgery, which was also statistically sig- nifieant（χ2 =6. 184,P=0.013）. Condusions miR-150-5P expression is downregulated in HCC, and is negatively correlated with lymph node metastasis and positively correlated with cumulative survival of HCC patients.