胡椒碱提取与纯化工艺的研究

Research on the Extraction and Purification Technology of Piperine

采用乙醇浸提法提取胡椒中的胡椒碱,通过单因素和L_(16)(4~5)正交实验,确定了最佳提取工艺条件:提取温度为65℃,乙醇浓度为75%,料液比为1∶30,提取时间为0.5h,提取次数为3次。通过静态和动态实验,确定了大孔树脂分离胡椒碱的最佳工艺条件:树脂种类为AB-8,上柱浸提液浓度为1.1mg/mL,pH值为6,洗脱液为体积分数75%、pH值为5的乙醇溶液,胡椒碱纯度由浸提液的16.46%上升到69.32%。最后,通过C_(18)层析柱对大孔树脂纯化的胡椒碱粗品进一步纯化,用90%乙醇洗脱,经HPLC测定纯化后粗品中的胡椒碱纯度上升为81.26%。

The piperine is extracted from pepper with ethanol extraction method, and the optimal extraction conditions are determined by single factor and L16 （45） orthogonal experiments, the ethanol concentration of 75%, the extraction temperature of 65℃, the solid-liquid ratio of 1：30, the extraction time of 0.5, the extraction times of 3. Based on the static and dynamic experiments, the optimal purification conditions for piperine separated by macroporous resins are obtained as follows. the type of macroporous resins of AB-8, the extract concentration of 1.1 mg/mL and the pH of 6, the eluted liquid of 75% （V/V） ethanol solution and pH of 5, the purity of piperine increases from 16.46% to 69. 32M. Finally, the crude product of piperine is purified by C18 column, and eluted by 90% （V/V） ethanol solution, and the purity of piperine increases to 81.26% measured by HPLC method.