崇明水仙种球贮藏期内源激素变化规律

Change Law of Endogenous Hormone During Storage Period of Chongming Narcissus(Narcissus tazettavar.chinensis) Ball

以崇明水仙种球为试材,采用ELISA法(酶联免疫法),研究了内源激素含量的变化规律对分别贮藏于20℃恒温和室温条件下,贮藏期内种球花芽分化的影响,以期为今后崇明水仙的花期调控等研究提供参考依据。结果表明:贮藏期间种球内的赤霉素(GA)含量均上升直至第7周,20℃处理组的峰值高出室温组40.67%。生长素(IAA)含量在2种贮藏条件下的变化略有差异,室温组IAA含量在第3~6周均高于20℃处理组,而20℃处理组的花芽分化进程在这4周中明显快于室温组。室温组玉米素核苷(ZR)含量变化与GA趋势一致,但20℃处理组的变化略有差异,直至取样结束,仍处于上升态势。2组处理的脱落酸(ABA)含量基本均呈现出先降后升的变化趋势,都在第3周出现最低值,室温组最低值低于20℃处理组16.97%,随后20℃处理组上升明显较室温组快。由此可知,相对低温可以加速水仙种球的花芽分化。20℃恒温贮藏对水仙花芽分化有促进作用,有利于GA含量的积累,且较高水平的GA含量可以加速花芽分化。高水平的ZR与促进花芽分化进程有密切关系。内源IAA和ABA水平则与花芽分化呈负相关。

The bulbs of Chongming narcissus（Narcissus tazetta var.chinensis）was used as test material,which were stored at 20℃and room temperature,respectively,were used to analyze the level of endogenesis hormones in bulbs for materials,by the method of ELISA（Enzyme-Linked Immuno Sorbent Assay）during storage.The related research results could be provided references for the study of flowering regulation of Chongming narcissus in the future.The results showed that GA content increased gradually up to the 7th week during storage,and the peak at 20℃ was higher than room temperature observed by 40.67%.IAA content was changed slightly different in the two storage conditions,IAA content at room temperature were higher than 20℃in the 3rd week to the 6th week,and the flower bud differentiation process of 20 ℃ was significantly faster than the room temperature in these four weeks.The change of ZR content in room temperature was consistent with the trend of GA,but slightly different changes in 20℃,until the end of the sampling,was still on the rise.ABA content in two groups showed the trend down first and up later,all appeared lowest value in the 3rd week,and the minimum at room temperature was lower than 20℃observed by 16.97%,then 20℃obviously rose faster than room temperature.Therefore,relatively low temperature could accelerate the flower bud differentiation of narcissus bulbs.20 ℃storage period could promote the narcissus flower bud differentiation,was conductive to the accumulation of GA content,and higher levels of the GA content could accelerate the flower bud differentiation.High levels of ZR had a close relationship with promoting the process of the flower bud differentiation.Endogenous IAA and ABA levels were negatively correlated with the flower bud differentiation.