大孔吸附树脂纯化桔梗茎总黄酮工艺及其抗氧化性

Purification Technology of Total Flavonods in Platycodon grandiflorum Stems With Macroporous Resin Methods and Its Antioxidant Ability

以桔梗茎为试材,采用单因素试验及正交实验方法,以总黄酮吸附率、解吸率为考察指标,研究了LSA-21型大孔吸附树脂纯化桔梗茎总黄酮的工艺条件及其体外抗氧化性。结果表明:桔梗茎总黄酮的最佳纯化工艺条件为样品液生药浓度0.06g·mL^(-1),样品液体积与树脂质量比值20.0mL·g^(-1),振荡吸附时间4.5h,70%乙醇溶液为洗脱剂,解吸液体积与树脂质量比值为27.5mL·g^(-1),振摇解吸时间3.5h,此条件下,桔梗茎浸膏中总黄酮的含量由6.37%提高到14.02%。桔梗茎总黄酮样品液对DPPH·及·OH具有较强的清除能力,而且其清除活性随桔梗茎总黄酮样品液质量浓度的增加而逐渐增强。

Platycodon grandiflorum stems were used as test materials.The purification technology of total flavonods（TFs）in stems with LSA-21 type macroporous resin methods and its antioxidant ability in vitro were discussed by single factor and orthogonal test.The results indicated that in the condition that the crude drug concentration of sample solution,ratio between sample solution volume and resin mass,batch adsorption time,concentration of eluent,ratio between desorption solution volume and resin mass and vibrating desorption time were 0.06g·mL-1,20.0 mL·g-（-1）,4.5hours,70% ethanol solution,27.5 mL·g-（-1） and 3.5hours,respectively,the content of TFs in Platycodon grandiflorum stems extracts increased from 6.37% to14.02%.The TFs in Platycodon grandiflorumstems had strong scavenging activity to DPPH·and·OH,and the scavenging activity might gradually be enhanced with the increase of mass concentration of TFs in extraction solution.