维生素C对顺铂治疗大鼠卵巢癌疗效的影响

The effect of vitamin C to cisplatin in the treatment of ovarian cancer rats

目的:探讨维生素C是否能影响顺铂对于大鼠卵巢癌的化疗效用,并对其作用机制进行初探。方法:建立卵巢癌大鼠模型,将大鼠随机分组后腹腔给药,每周观察一般状况变化。8周后,脱颈处死各组大鼠,取血清检测CA125、HE4并计算ROMA值,测量腹水容积,解剖并观察统计肿瘤生长情况,对局部肿块及主要组织进行测量,常规病理学检查。结果:所有大鼠外观消瘦,体重随着腹水和肿瘤的出现呈现增长,顺铂+高剂量维生素C组的肿瘤质量及腹水容积明显小于其余三组,实验组的CA125、HE4及ROMA明显低于对照组,顺铂+高剂量维生素C组的CA125、HE4及ROMA则明显低于顺铂+低剂量维生素C组及顺铂组,实验组患恶性肿瘤的大鼠比例降低,而是否添加维生素C对数据则无明显影响。结论:注射较高浓度维生素C能增强顺铂杀伤卵巢癌的能力,并呈现一定的剂量依赖性。

Objective：To investigate whether vitamin C can affect the effectiveness of cisplatin chemotherapy for o-varian cancer rats,and explore its mechanism. Methods：To build modeling ovarian cancer in rats,after the rats were randomized intraperitoneal administration was opearted,observed general condition weekly. After 8 weeks,the rats were sacrificed,then detected the serum CA125,HE4 and calculated ROMA value,measured ascites volume,dissected and recorded tumor weight,statistics for major organizations and local mass measurements,with routine pathology ex-amination. Results：All rats showed emaciated appearance. Weight gained because of tumors and ascites. Tumor weight and ascites volume of cisplatin ＋ high - dose vitamin C group were significantly smaller than the other three groups. CA125,HE4 and ROMA value of three experimental groups were significantly lower than the control group,CA125, HE4 and ROMA value of cisplatin group ＋ high - dose vitamin C group significantly lower than cisplatin ＋ low - dose vitamin C group and cisplatin group. Proportion of cancer cases in the experimental group reduced,and whether vita-min C added had no significant effect on the data. Conclusion：The injection of high concentrations of vitamin C can enhance the anti - ovarian cancer ability of cisplatin,which in a dose - dependent manner.