PM_(2.5)对大鼠气管上皮细胞氧化应激及自噬的影响

The effects of PM_(2.5) on oxidative stress and autophagy of tracheal epithelial cell

目的:探讨PM_(2.5)对RTE大鼠气管上皮细胞的氧化应激及自噬的影响。方法:采集制备不同浓度的PM_(2.5)来处理RTE细胞,用MTT比色法测定细胞增值率,流式细胞术检测细胞内活性氧类(ROS)自由基生成,Western Blot检测微管相关蛋白1轻链3I蛋白(LC3I)和LC3II表达。结果:PM_(2.5)100mg/L和200mg/L处理后细胞存活率均明显降低,并呈浓度和时间依赖性。不同浓度PM_(2.5)可升高胞内ROS水平及增加LC3II蛋白表达,亦呈浓度和时间依赖性。结论:PM_(2.5)通过诱发氧化应激及自噬对气道上皮起到损害作用,其中氧化应激促进细胞过度自噬的发生。

Objective ：To study the effects of PM 2 .5 on oxidative stress and autophagy of tracheal epithelial cell .Methods ：After RTE cells were treated by different concentrations of PM 2 .5 collected and prepared ,cell incre‐ment rate was determined by M TT colorimetric method ,whether free radical generation of intracellular reactive oxygen species （ROS） was detected with flow cytometry , whether microtubule associated protein light 1 chain 3 I protein 1 （LC31） and LC3II was expressed with Western Blot .Results ：After treated by PM 2 .5 100 mg/L and 200 mg/L ,Cell survival rate were significantly decreased with concentration and time dependence ,PM 2 .5 can also increase the intracellular ROS level and LC3 II protein expression with concentration and time dependence .Conclusion ：Airway epithelia cells may be damaged by PM 2 .5 through oxidative stress and autophagy in which course oxidative stress could promote cell excessive autophagy .