胸腺素β4在结肠息肉和结肠癌中表达及其意义

Expression and Significance of Thymosin β4 in Colon Polyps and Colon Cancer

目的探讨胸腺素β4在结肠息肉和结肠癌中表达及其意义。方法选取2015年7月—2016年7月在该院治疗的结肠息肉和结肠癌患者67例,根据患病类型分为结肠息肉组28例,结肠癌组28例,结肠息肉+结肠癌组11例。分别取标本(手术切除或息肉摘除)和距切除标本15 cm以上正常肠粘膜组织,提取其m RNA行RT-PCR,通过凝胶扫描仪进行各电泳条带和光量子强度分析,测定各标本中胸腺素β4的相对含量;抽取患者血清,用胸腺素β4酶联免疫吸附试剂盒检测患者血清中胸腺素β4在息肉和肠癌切除术前、术后1月及术后6个月的表达情况。结果胸腺素β4的m RNA表达肠息肉、肠癌患者中,均有胸腺素β4m RNA的表达;且相对其正常肠组织中含量[结肠息肉组(0.41±0.17)、结肠癌组(0.50±0.14)],其病理标本中m RNA均有高表达[结肠息肉组(0.59±0.22)、结肠癌组(0.75±0.23)],P<0.05,差异有统计学意义;结肠息肉组切除术前、术后1个月及术后6个月,胸腺素β4的表达,P>0.05,差异无统计学意义;结肠癌组和结肠息肉+结肠癌组切除术前、术后1个月血清胸腺素β4的表达有显著变化,P<0.05,差异有统计学意义,而术前、术后6月则无显著变化,P>0.05,差异无统计学意义。结论胸腺素β4在息肉及肠组织中均有高表达,可作为观察肠癌疗效的参考指标。

Objective To investigate the expression and significance of thymosin β4 in colon polyps and colon cancer.Methods 67 cases of colon polyps and colon cancer patients treated in our hospital from July 2015 to July 2016 were selected.According to the type of disease,28 cases were divided into cases of colonic polyps,28 cases of colon cancer group,11 cases of colon polyps and colon cancer group.The specimens were（resection or polyp） and from resected specimens of 15 cm above the normal intestinal mucosa,extracted the m RNA useing RT-PCR analysis the electrophoretic bands and photon intensity through the gel scanner,the relative content of each sample in the determination of Serum thymosin β4.The expression of thymosin β4 in patients with polyps and colorectal cancer before 、after 1 month and after 6 months of operation was detected by using the serum of patients with thymus peptide and 4 enzyme linked immunosorbent assay.Results m RNA expression of thymosin β4 of intestinal polyps,colorectal cancer patients,all have the expression of the m RNA of thymosin β4,The expression of m RNA in the pathological specimen was higher[colonic polyps（0.59±0.22）、colon cancer group（0.75±0.23）] than those of normal intestinal tissue content[（colonic polyps（0.41±0.17）、colon cancer group（0.50±0.14）],P 〈0.05,the difference was stat istically significant;the difference between the expression of thymosin β4 in before 、after 1months and after 6 months in the colonic polyps was no statistically significant;the expression of thymosin β4 before the operation in the colon cancer group and colon polyps and colon cancer group was higher than those of after the operation 1 month,P〉0.05,the difference was statistically significant;difference between the expression of thymosin β4 before the operation and after the operation 6months was no statistically significant,P〉0.05.Conclusion Thymosin β4 was highly expressed in the polyp and intestinal tissue,and serum thymic peptide 4 could be used as a reference index for the observation of the curative effect of colorectal cancer.