酶联免疫吸附结合免疫亲和柱-高效液相色谱法检测大米中黄曲霉毒素B_1的研究

Study on Determination of Aflatoxin B_1 in Rice by ELISA and IAC-HPLC

对大米中两种检测黄曲霉毒素B1方法的结合使用进行了研究,即采用酶联免疫吸附法对大米中黄曲霉毒素B1进行初筛,对可疑样品采用免疫亲和柱-高效液相色谱法进行验证。样品经甲醇水提取、免疫亲和柱净化、高效液相色谱分离、碘柱后衍生、荧光检测器测定。结果表明,黄曲霉毒素B1线性关系良好,相关系数为0.9999,检出限为0.50μg/kg,回收率为89.4%~95.2%,RSD值为1.88%~3.05%。两种方法的结合应用,适用于较大批量样品的检测。

The combination of two detection methods of aflatoxin B_1 in rice was studied. Aflatoxin B1 in rice were screened by ELISA,the suspicious samples were verified by IAC-HPLC. Samples were extracted with methanol-water solution and cleaned up by immune-affinity columns,separated by HPLC,and the determination was carried out by FLD after iodine derivatization. The method had good linearity and the correlation coefficients was 0. 9999,the limit of detection was 0. 50 μg / kg. The average recoveries was in the range of 89. 4 %~ 95. 2 % with relative standard deviations（ RSD） of 1. 88 % ~ 3. 05 %. The combination of the two methods is suitable for the detection of large quantities of samples.