不同提取方法对姬菇多糖抗氧化活性的影响

The Effect of Antioxidant Activity on the Polysaccharides from Pleurotus cornucopiae with Different Extraction Ways

本研究采用传统水提法、超声波提取法、超声波辅助水提法、酸提法、碱提法、超声波辅助酸提法和超声波辅助碱提法7种方法提取姬菇多糖,测定还原力、羟自由基(·OH)清除率、超氧阴离子自由基(O_2^-·)清除能力、DPPH自由基清除力作为体外抗氧化作用的评价指标,并与Vc对照.结果表明酸法提取多糖得率最高,可达6.58%.总体来看,超声波辅助水提法所得姬菇多糖的抗氧化活性最好,多糖浓度为10 mg·m L^(-1)时其还原力达到2.18,·OH清除率为95.56%;多糖浓度为1 mg·m L^(-1)时,O_2^-·的清除率达到62.35%;多糖浓度为2 mg·m L^(-1)时,对DPPH自由基的清除率达到80.32%.超声波辅助碱提法所得多糖的抗氧化活性也较好,而超声波辅助酸提法所得多糖的抗氧化活性最差.由此可见,采用超声波辅助水提法所得多糖有利于姬菇多糖生物活性方面的研究.

Seven different extaction methods of the polysaccharides from pleurotus cornucopiae, including traditional hot water extraction, ultrasonic extraction, ultrasonic wave complex hot water extraction, acid extraction, alkali extraction, ultra-sonic wave complex acid extraction and ultrasonic wave complex alkali extraction. And antioxidant activity of the polysac-charides in virto was investigated by using reducing power, hydroxyl radical scavenge activity,superoxide radical assay and DPPH＊ scavenge activity, using Vc as positive control. The results showed that the polysaccharides prepared by acid extrac-tion had the highest extraction yield that reached 6.58%. In general,the polysaccharides by ultrasonic wave complex hot water extraction reached the highest reducing power（2.18 at 10 mg＊mL 1） , hydroxyl radical scavenging ability was（95.56% at 10 mg＊mL l ） , Superoxide anion radical scavenging activity was（62.35% at 1 mg＊mL l ） ,DPPH＊ scavenging ability was （80.32% at 2 mg ？ mL l） . The antioxidant activity of the polysaccharides prepared by ultrasonic wave complex alkali extraction was also strong, but the polysaccharides prepared by ultrasonic wave complex acid extraction was the worst. It is concluded that ultrasonic-assisted hot water extraction is an effective method for extracting polysaccharides from pleurotus cornucopiae, and it＇s more conducive to study the biological activity of polysaccharides.