暗纹东方鲀生长性状相关微卫星标记筛选

Screening growth-related microsatellite markers in Takifugu obscurus

为了筛选与暗纹东方鲀(Takifugu obscurus)生长相关的分子标记,作者采用SSR结合BSA技术对同龄孵化群体的同池养殖暗纹东方鲀生长差异性状标记进行筛选。利用85对微卫星引物对暗纹东方鲀生长快、慢各30尾个体构建的两个基因池进行分析,共筛选到14个差异位点。然后分析这14个差异微卫星位点在这60个暗纹东方鲀个体中的基因型差异,结果表明:位点TOP03、TOG01、fms15、fms75与生长性状呈现极显著负相关关系(P<0.01),fms89与生长性状呈现极显著正相关关系(P<0.01)。用另外30个个体(生长快慢各15个)进行验证实验后,结果只有位点fms15、fms75与生长性状显著相关,相关系数分别为–0.411和–0.384。这两个微卫星位点对于暗纹东方鲀生长性状有显著效应,为开展暗纹东方鲀的分子标记辅助育种提供了有价值的参考标记。

A population of Takifugu obscurus with significant growth differences was investigated for screening molecular markers linked to growth traits, using SSR combined with bulked segregation analysis（BSA）. Two gene pools were respectively constructed using 30 DNA templates isolated from T. obscurus samples with significantly different growth rates and then amplified by 85 pairs of microsatellite primers. The unique alleles were found in 14 loci. These 60 T. obscurus individuals were genotyped by SSRs. The results showed that loci TOP〈03, TOG01, fms15, and fms75 had a highly significant negative correlation with growth trait（P〈0.01）, while fms89 showed extremely significant positive correlation with growth trait（P〈0.01）. The verification test using another population of 30 randomly selected T. obscurus with significantly different growth rates indicated that only loci fms15 and fms75 were significantly correlated with growth trait, with correlation coefficients of –0.384 and –0.411, respectively. Therefore, these two microsatellite loci with a significant linkage with growth traits could be used as reference markers for marker-assisted breeding of T. obscurus.