中国南海圆盘肉芝软珊瑚萜类成分研究

Terpenes from South China Sea soft coral Sarcophyton trocheliophrum

目的研究采自中国南海的圆盘肉芝软珊瑚的萜类成分。方法采用1H-NMR导向分离法,使用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱（HPLC）等方法进行化学成分分离,根据质谱（MS）、核磁共振（NMR）等波谱数据鉴定化合物结构。结果从圆盘肉芝软珊瑚的丙酮提取物的乙醚萃取部位中分离得到9个萜类化合物,分别鉴定为瑞士松烯A（1）、（E,E,E）-7,8-epoxy-1-isopropyl-4,8,12-trimethylcyclotetradeca-1,3,11-triene（2）、sarcophytonolide A（3）、deacetylemblide（4）、4Z,12Z,14E-sarcophytolide（5）、sarcrassin D（6）、emblide（7）、（4Z,8S,9R,12E,14E）-9-hydroxy-1-isopropyl-8,12-dimethyloxabicyclo[9.3.2]-hexadeca-4,12,14-trien-18-one（8）、β-榄香烯（9）;其中化合物1~8为二萜类,化合物9为倍半萜类。结论化合物4是1个新的天然产物,并首次报道其13C-NMR数据。化合物1、3、5、8为首次从该种软珊瑚中分离得到。生物活性研究结果表明,化合物5对人蛋白酪氨酸酶（PTP1B）具有中等强度的抑制作用（IC50=15.4μmol/L）。

Objective To investigate the terpenes of the South China Sea soft coral Sarcophyton trocheliophrum. Methods Subjected to 1H-NMR-guided fractionation, the chemical constituents were isolated and purified using column chromatographies on silica gel, Sephadex LH-20, and high performance liquid chromatography（HPLC）. Their structures were determined through the analysis of spectroscopic data. Results Eight diterpenes and one sesquiterpene were isolated from the ethyl ether soluble part of acetone extract of S. trocheliophrum and their structures were identified as cembrene A（1）,（E,E,E）-7,8-epoxy-1-isopropyl-4,8,12-trimethylcyclotetradeca-l,3,11-triene（2）, sarcophytonolide A（3）, deacetylemblide（4）, 4Z,12 Z,14E-sarcophytolide（5）, sarcrassin D（6）, emblide（7）,（4Z,8S,9R,12 E,14E）-9-hydroxy-1-isopropyl-8,12-dimethyloxabicyclo [9.3.2]-hexadeca-4,12,14-trien-18-one（8）, and β-elemene（9）, respectively. Conclusion Compound 4 is found to be a new natural product, and its 13C-NMR data are recorded for the first time. Moreover, this is the first report that compounds 1, 3, 5, and 8 are obtained from the title soft coral. In bioassay, compound 5 exhibits the moderate human protein tyrosine phosphatase 1B（PTP1B） inhibitory activity（IC50 = 15.4 μmol/L）.