摘要
分离克隆了猪MBF1基因第4外显子的序列,测序发现在18 bp处存在T/C突变,引起Taq I酶切多态。利用PCR-RFLP方法对国内外4个猪种MBF1基因第4外显子的多态性进行分析发现,在国外猪种中等位基因T的的频率占优势;在中国猪种中C等位基因的频率占优势;在437头大梅F2代资源家系中进行性状关联分析发现该多态与瘦肉率、板油重、肩部背膘厚、眼肌高、眼肌宽、眼肌面积显著相关(P<0.05)。
Cloning and Sequencing of the 4thexon of porcine MBF1 gene indicated that a T18 C substitution which can be detected by restriction endonuclease Taq I. This polymorphism was analyzed with PCR-RFLP among four foreign and Chinese pig breeds. Analysis result showed that allele T was predominant in foreign pig breeds;however allele C was predominant in Chinese pig breeds. Statistical analysis in 437 Large White×Meishan F2 progeny pigs showed that the Taq I polymorphism was associated with lean meat percentage,leaf fat weigh,shoulder fat thickness,loin eye height,loin eye width,loin eye area,significantly（P0.05）.
出处
《湖北农业科学》
2016年第23期6192-6194,6287,共4页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心项目资助(2016-620-004-001)
国家生猪产业技术体系(CARS-36)
国家科技支撑计划项目(2011BAD28B01-13)
湖北省重大人才工程高端人才引领培养计划
湖北省农业科学院青年基金项目(2016NKYJJ36)
