微小RNA-126对卡波西肉瘤细胞迁移和侵袭能力的影响

Influence of microRNA-126 on migration and invasion of human Kaposi’s sarcoma cell line

目的探讨卡波西肉瘤细胞系转染微小RNA-126(microRNA-126,miR-126)后对细胞迁移和侵袭能力影响。方法卡波西肉瘤细胞株SLK随机分为miR-126模拟物组、阴性对照组、miR-126抑制物组和抑制物阴性对照组,分别将miR-126模拟物25ng、阴性对照25ng、miR-126抑制物250ng和抑制物阴性对照250ng加入转染试剂3μL,室温孵育10min形成转染混合物,分别转染到4组细胞悬液中,孵育48h后行Transwell小室迁移实验和侵袭实验,将Transwell小室基底膜完整切除,行苏木精染色,光学显微镜下计数细胞迁移数目和细胞侵袭数目。结果 miR-126模拟物组细胞迁移数目[(77.33±7.02)个]和细胞侵袭数目[(25.67±2.52)个]较阴性对照组[(96.67±6.11)、(45.67±2.08)个]、miR-126抑制物组[(138.00±8.00)、(76.67±4.16)个]和抑制物阴性对照组[(101.33±9.29)、(47.33±1.53)个]少(P<0.05);miR-126抑制物组细胞迁移和侵袭数目较阴性对照组和抑制物阴性对照组多(P<0.05);阴性对照组细胞迁移和侵袭数目与抑制物阴性对照组比较差异无统计学意义(P>0.05)。结论 miR-126对卡波西肉瘤细胞侵袭和迁移起抑制作用,可能成为卡波西肉瘤的潜在治疗靶点。

Objective To investigate the influence of microRNA-126 （miR 126） transfection on the migration and invasion of Kaposi＇s sarcoma cell line. Methods Kaposi＇s sarcoma cell line SLK was randomly divided into miR-126 mimic group （25 ng）, negative control group （25 ng）, miR-126 inhibitor group （250 ng） and inhibitor negative control group （250 ng）, which were added 3 μL transfection reagent, and incubated for 10 min at room temperature. The mixture was transfected into Kaposi＇s sarcoma cell suspension. After 48-h incubation, Transwell experiment and invasion experiment were performed, and the Transwell cell basement membrane was completely removed. All groups received hematoxylin staining. The numbers of migration and invasion cell were counted under optical microscope. Results The numbers of migration cells （77.33±7.02） and invasion cells （25.67±2.52） in miR-126 mimic group were obviously less than those in negative control group （96.67±6.11, 45.67±2.08）, miR-126 inhibitor group （138.00±8.00, 76.67± 4.16）, and inhibitor negative control group （101.33±9.29, 47.33±1.53） （P〈0.05）. The numbers of migration cells and invasion cells were significantly more in miR-126 inhibitor group than those in negative control group and inhibitor negative control group （P〈0.05）, and there was no significant difference between negative control group and inhibitor negative control group （P〉0.05）. Conclusion MiR-126 could inhibit the invasion and migration of Kaposi＇s sarcoma cell and could become a potential therapeutic target of Kaposi＇s sarcoma.