基于金纳米颗粒负载的金属有机骨架材料的癌胚抗原传感器的研制

Preparation of Carcinoembryonic Antigen Immunosensor Based on Au Nanoparticles Loaded-Metal-Organic Frameworks

以负载Au的金属有机骨架材料(Au NPs/Cu-TPA)标记CEA抗体(Ab2)为信号探针,通过电还原的方法将氧化石墨烯还原到电极上,研制了一种捕获CEA抗体(Ab1)的电化学免疫传感器,并将其应用于癌胚抗原(CEA)检测。所合成的MOFs材料中含有大量Cu2+,且电化学信号比较稳定,因此可以通过检测MOFs材料中Cu2+的信号实现对CEA的检测。此信号探针不需要预处理和酸处理,易负载贵金属从而固定抗体,大大简化了检测步骤并缩短了检测时间。此传感器对CEA的检测灵敏度好,操作简便。在最优实验条件下,此传感器的线性范围为0.1~80 ng/m L,检出限为0.03 ng/m L,线性相关系数为0.9887,可用于真实样品中CEA的测定。

A novel and simple electrochemical immunoassay for carcino-embryonic antigen（CEA） was developed using Au nanoparticles loaded-metal-organic frameworks（Au NPs/Cu-TPA） as signal unit and electrochemical reduction of graphene oxide on the surface of electrode to capture CEA antibody（Ab1）.MOFs contain large amounts of Cu^2＋ions,which can be used as stable electrochemical signals to achieve the detection of CEA.This new class of signal probe differs from traditional probe because it does not require pretreatment and acid treatment,and easy to load noble metal for the immobilization of antibody,which will greatly simplify the detection steps and reduce the detection time.This immunosensor has good sensitivity and ease to operate.Under the optimized experimental conditions,the proposed sensing strategy provides a linear dynamic range from 0.1 ng/m L to 80 ng/m L with a detection limit of 0.03 ng/m L and linear correlation coefficient of 0.9887.The developed CEA immunosensor can be used to detect CEA in real sample.