夹心法免疫层析试条的数学模型与仿真研究

Study on Mathematical Model and Simulation of Sandwich-type Lateral Flow Immunoassay

基于夹心法免疫层析试条检测原理,结合对流扩散方程和流体动力学方程,建立了夹心法免疫层析试条动态反应过程的数学模型,并通过COMSOL软件对试条动态反应过程进行仿真。分别探究了目标待测物A浓度在0~20 mol/L,标记物P浓度在1×10!2~1×103mol/L以及硝酸纤维素膜的孔隙率在0~1范围内变化时,检测线上夹心复合物浓度关于位置和时间的浓度变化情况,并分析了各物质初始浓度以及试条结构对于检测结果和检测时间的影响。结果表明,在一定浓度范围内,目标待测物A以及标记物P浓度的增加将提高试条的定量检测性能,而孔隙率通过影响混合液流速和混合液中各物质反应接触情况来影响检测结果。

A mathematical model of sandwich-type lateral flow immunoassay（LFIA） is established to describe the dynamic process of LFIA according to the biochemical principle of LFIA together with the convection-diffusion equations and the hydromagnetic equations.Based on the established model,the COMSOL software is utilized to simulate the dynamic process of LFIA.The simulation results not only demonstrate the relationships between the concentration of the sandwiched substance and the position or the time,but also analyze the influences of the initial concentrations of all substances and the structure on the performance of LFIA system when the target analyte A is from 0 to 20 mol/L,reporter particle P is from 1 ×10!2mol/L to 1 × 103 mol/L and the porosity is from 0 to 1.Especially,within a certain concentration range,the increasing concentration of target analyte A and reporter particle P will enhance the quantitative performance of LFIA,and the porosity affects the result by controlling the flow rate of the test mixture and the material contact in the mixture.