高效液相色谱法测定化妆品中22种紫外吸收剂

Determination of 22 kinds of ultraviolet absorbent by high performance liquid chromatography in cosmetics

建立了一种高效液相色谱同时测定防晒类化妆品中22种紫外吸收剂的方法。以四氢呋喃或V(四氢呋喃)∶V(水)∶V(甲醇)=9∶6∶5的混合溶液提取化妆品中的紫外吸收剂,以Agilent C_(18)色谱柱进行分离,以甲醇-高氯酸水溶液-四氢呋喃-乙腈混合液为流动相进行梯度洗脱,流速1.0 m L/min,在检测波长311 nm处进行测定。结果表明,22种紫外吸收剂的色谱峰实现良好分离,线性范围为0.1~500 mg/L,相关系数均大于0.999,定量限均不高于4.6 mg/L,加标回收率为85.6%~101.0%。建立的方法线性范围宽、定量限低、灵敏度高、结果准确可靠,能够满足《化妆品安全技术规范》(2015年版)对紫外吸收剂的检测要求。

An analytical method for simultaneous detection of 22 kinds of ultraviolet （UV） absorbent in sunscreen cosmetics was established by using HPLC. After the cosmetic samples were extracted by tetrahydrofuran （THF） or mixed solvent with V （THF） ： V （water） ： V （ methanol ） = 9 ： 6 ： 5, the extracted components were separated using Agilent Cls column with gradient elution by a mixed solvent composed of methanol - perchloric acid aqueous solution - THF - acetonitrile with flow rate of 1.0 mL/min, and detected by a diode array detector （DAD） at 311 rim. The results showed that 22 kinds of UV absorbent were separated and detected well. The linearity relationship is within 0. 1 - 500 mg/L. The correlation coefficient is greater than 0. 999. The limit of quantification （LOQ） of all kinds of the UV absorbent is not higher than 4. 6 mg/L. The spiked recovery rate is between 85.6% and 101.0%. In conclusion,the method was accurate and reliable with wide linear range, low LOQ and high sensitivity, which satisfies the requirement specified in China Safety and Technical Standards for Cosmetics （2015 edition）.