花生AhFUSCA3基因的原核表达及在非生物胁迫下的表达分析

Prokaryotic Expression of AhFUSCA3 Gene and Its Expression Analysis under Abiotic Stresses in Peanut

根据本实验室已获得的花生AhFUSCA3基因(NCBI登录号JX420284)序列,设计特异引物,构建原核表达载体,进行了重组蛋白表达分析,获得了分子量为42KD左右的目的蛋白条带;利用荧光定量PCR(Quantitative Real-time RT-PCR)方法,检测了AhFUSCA3基因在低温、高盐和干旱胁迫条件下的表达情况。结果显示,AhFUSCA3基因在低温和高盐处理的花生叶片中表达量明显上调,但在干旱处理的叶片中表达量则有明显下降。以上结果表明AhFUSCA3基因可能参与了花生对低温、高盐和干旱的抗性调控。

In this study,according to the sequence of AhFUSCA3gene（GenBank accession number JX420284）,specific primers were designed to construct prokaryotic expression vector.The recombinant protein was induced and the molecular weight of the target protein was about 42 KD.Expression analysis indicated that the AhFUSCA3 gene was induced in peanut leaves under either cold or salt conditions.However,the expression of the AhFUSCA3 gene was decreased in leaves under drought treatment.The results suggested that the AhFUSCA3 gene may be involved in abiotic stress regulation of peanut.