溃疡性结肠炎患者结肠黏膜中胸腺基质淋巴细胞生成素的表达水平

Expression of thymic stromal cells in colonic mucosa of patients with ulcerative colitis

目的研究溃疡性结肠炎(UC)患者结肠黏膜中胸腺基质淋巴细胞生成素的表达水平及其与病变范围、病变程度间的关系,初步探讨UC的发病机制。方法 34例UC住院患者作为研究组,29例结肠息肉患者作为对照组,采用实时定量PCR法测定两组结肠黏膜组织中TSLP mRNA表达水平,比较血清CRP及血沉的变化。结果研究组与对照组结肠黏膜TSLP mRNA表达水平及CRP、血沉存在显著差异(P<0.01);UC患者中结肠黏膜TSLP mRNA表达水平、CRP以及血沉在不同病变程度及不同病变部位间存在显著性差异(P<0.01);UC患者TSLP mRNA表达水平与CRP、血沉之间存在显著正相关(P<0.01),TSLP mRNA表达水平与病变程度间存在显著正相关(P<0.01)。结论与正常人比较,UC患者结肠黏膜中TSLP表达水平明显升高,结肠黏膜TSLP表达水平与UC病变程度和范围存在显著相关性。

Objective To explore the expression level of thymic stromal lymphopoietin（TSLP） in colonic mucosa and its relationship with the extent and degree of the lesions in patients with ulcerative colitis.Methods A total of 34 patients with ulcerative colitis were selected as the research group, 29 patients with colonic polyps were selected as the control group. Real-time quantitative PCR was used for the determination of TSLP mRNA expression level of colon mucosa. The different degree of C reactive protein（CRP） and the change of erythrocyte sedimentation rate（ESR） were compared. The correlation of TSLP mRNA expression level, CRP and ESR in patients with ulcerative colitis was analyzed. Results The TSLP mRNA expression level of colonic mucosa, CRP, and ESR between the research group and control group had significant difference（P〈0.01）. In UC patients, TSLP mRNA expression level of colonic mucosa, CRP and ESR among different lesion degree and lesion distribution had significant difference（P〈0.01）. TSLP mRNA expression level of colonic mucosa, CRP and ESR in UC patients had significant positive correlation（P〈0.01）. TSLP mRNA expression levels and the extent of lesions had a significant positive correlation（P〈0.01）. Conclusions Compared with normal persons, the expression of TSLP of colon mucosa in UC patients was significantly increased,and the expression level of TSLP was significantly correlated with the lesion degree and lesion distribution.