大黄酚脂质体对脑缺血再灌注损伤小鼠动态抗氧化作用研究

Study on Dynamic Anti-oxidative Effects of Chrysophanol Lipidosome on Cerebral Ischemia-reperfusion Injury in Mice

目的:探讨大黄酚脂质体对脑缺血再灌注损伤小鼠动态抗氧化作用。方法:采用线栓法制备脑缺血再灌注模型,小鼠随机分为空白对照组、假手术组、模型组、尼莫地平组(nimodipine,1.0 mg·kg-1)、大黄酚脂质体组(10.0、5.0、0.5 mg·kg-1)。空白对照组、假手术组和模型组腹腔注射(intraperitoneal injection,ip)生理盐水(10 m L·kg-1),大黄酚脂质体组在缺血再灌注前1 h给予药物,并于缺血后给予药物,一天一次,每组存活小鼠按观察时间点随机分为3、6、12、24、48、72 h组,每组15只。于规定时间观察小鼠神经功能损伤评分,测定大脑含水量、脑梗死面积、以及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、一氧化氮合酶(nitric oxide synthase,NOS)的活性和丙二醛(malonaldehyde,MDA)、一氧化氮(nitric oxide,NO)含量。结果:与假手术组比,模型组神经功能损伤评分、大脑含水量、脑梗死面积、MDA、NO含量,NOS活性显著增加(P<0.01),SOD活性,GSH-PX活性显著降低(P<0.01);大黄酚脂质体组显著降低神经功能损伤评分、大脑含水量、脑梗死面积、MDA含量,NO含量,NOS活性,显著增强SOD活性,GSH-PX活性(P<0.05)。结论:大黄酚脂质体可能通过抗氧化实现其对小鼠脑缺血再灌注损伤的保护作用。

Objective：To explore the dynamic anti-oxidative effects of chrysophanol lipidosome on cerebral ischemia-reperfusion injury in mice.Methods：Mice were subjected to middle cerebral artery occlusion by suture method,then they were randomly divided into the control group,sham group,model group,nimodipine group（1.0 mg·kg-1） and chrysophanol lipidosome treatment（10.0,5.0,0.5 mg·kg-1） groups. The control group,sham group,model group were injected with normal saline（10 mL·kg-1,ip）. Chrysophanol lipidosome treatment groups intraperitoneally were injected with chrysophanol lipidosome（10.0,5.0,0.5 mg·kg-1） before cerebral ischemia one hour,once a day,then the mice were divided into 6 subgroups according to different reperfusion time points,3,6,12,24,48,72 hour. According to the different time points,neurological deficits,brain water content,infarct volume were determined;the activity of NOS,SOD,GSH-PX,the content of MDA,NO were determined by the kits methods. Results：Compared with sham group,in model group the neurological deficits,brain water content,infarct volume were increased（P〈0.01）;the activity of NOS and the content of MDA, NO were increased;the activity of SOD and GSH-PX were reduced（P〈0.01）. Chrysophanol lipidosome reduced the neurological deficits,brain water content,infarct volume,the content of MDA,NO,and activity of NOS,and elevated the activities of SOD,GSH-PX（P〈0.05）. Conclusion：So the protective effect of chrysophanol lipidosome on cerebral ischemia-reperfusion injury might be involved in its anti-oxidative.