2’-溴-2-羟基-4,6,4’,5’-四甲氧基查尔酮抑制SK-N-SH细胞增殖的作用研究

Antiproliferation Effects of 2'-bro-2-hydroxy-4,6,4',5'-tetrametho-xychalcone on Human Neuroblastoma SK-N-SH Cells

目的：研究2’-溴-2-羟基-4，6，4’，5’-四甲氧基查尔酮（2’-bro-2-hydroxy-4，6，4’，5’-tetrametho-xychalcone，C30）的抗人神经母细胞瘤细胞（SK-N-SH）作用及其机制，为查尔酮类新型化合物开发用于神经母细胞瘤的预防和治疗提供初步的实验依据。方法：磺酰罗丹明B（sulforhodamine B，SRB）法检测药物作用肿瘤细胞后的细胞活性；流式细胞术检测细胞周期和凋亡；Hoechst 33258染色法检测细胞凋亡。结果：C30在1.5625~25.0000μmol·L-1的浓度范围内对SK-N-SH细胞有较强的抑制作用，抑制效应在此范围内呈剂量和时间依赖性。且C30使SK-N-SH细胞的周期阻滞于G1期，并可以诱导SK-N-SH细胞发生凋亡。结论：查尔酮类新型化合物C30能抑制SK-N-SH细胞增殖，作用机制可能与其改变SK-N-SH细胞周期分布和诱导细胞凋亡相关。

Objective：We studied the anti-proliferation effects of 2’-bro-2-hydroxy-4, 6,4’,5’-tetrametho-xychalcone（C30） on human neuroblastoma and its mechanism to provide preliminary experimental evidence for its potential application to the prevention and treatment of neuroblastoma.Methods：The antiproliferation effects of the compounds on tumor cells were detected by sulforhodamine B（SRB） assay. Cell cycle distributions were measured by flow cytometry. Cellular apoptosis was detected by Hoechst 33258 staining assay and flow cytometry. Results：C30 restrained the proliferative effect of SK-N-SH cells in a dose- and time- dependent manner. After treated with C30,the cell cycle of SK-N-SH was arrested in G1 phase. Hoechst 33258 staining and flow cytometry analysis revealed that petasin could induce apoptosis in SK-N-SH cells.Conclusion：C30can inhibit the proliferation of SK-N-SH,and the mechanism may be related to changing cell cycle distribution and inducing apoptosis.