基于ITS条形码序列的枸杞属植物鉴定

Identification of Lycium L. Germplasm Resources Based on nrDNA ITS Sequence

采用改进CTAB法提取枸杞（Lycium Chinense Mill.）叶片DNA,利用合成的特异引物对其DNA中nr DNA ITS区进行扩增,利用ITS条形码序列,对枸杞属种质资源进行鉴定,分析其亲缘关系。结果表明,测序得到了17份枸杞属近缘种的ITS条形码序列,整个ITS序列长度变异范围为603~632 bp,平均为624 bp,整个转录间隔区（ITS1＋ITS2）对位排列后总长度为480 bp,有194个变异位点,占40%;保守位点288个,占60%。聚类分析结果表明,17份种质资源可分为5个大类群。基于ITS条形码序列分析在鉴定枸杞属种质遗传多样性及其亲缘关系具有一定的优越性。

Adopting improved CTAB to acquire DNA of leaf of Lycium Chinense Mill.,and making use of composite special primers to propagate and clone nr DNA ITS area of DNA,and then sequencing analysis of the target segment. To identify genetic diversity of Lycium L. germplasm resources by the nr DNA ITS sequence. The results showed that the nr DNA ITS sequence of 17 Lycium L. was gained for the first time;the variation length of the whole ITS sequence was 603 to 632 bp,and the average was 624 bp; the total length of whole transcribe partition area（ITS1 and ITS2） sequence alignmentwas 480 bp,and there were 194 variable sites accounting for 40.4%, and 286 conserved sitesaccounting for 59.6%. Based on the nr DNA ITS sequence analysis of 17 Lycium L. germplasm resources,it was obvious that they could be divided into 5 groups. The nr DNA ITS sequence analysis is a advantageous way to study genetic diversity among Lycium L. germplasm resources.